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Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes
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Medicine
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JoVE Journal Medicine
Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes

DOI:
10.3791/63329-v

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11:47 min

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March 04, 2022

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, , ,
1Department of Medicine, Division of Nephrology,Indiana University School of Medicine

Chapters

  • 00:05Introduction
  • 00:55Preparing the Munich Wistar Frmter or SD Rat for Unilateral Ureteral Obstruction (UUO) Surgery
  • 01:53Surgical Preparation for Intravital 2-Photon Imaging
  • 04:04Positioning the Rat for Imaging and Image Acquisition for Quantitative Analysis
  • 06:16Calculating Red Blood Cell Flow in Surface Glomerular Capillary Loops and Renal Vasculature Using a Linescan Function
  • 07:45Calculating White Blood Cell (WBC) Occlusion in Glomerular Capillary Loops
  • 08:38Results: Quantification of Glomerular Processes in the Unilateral Ureteral Obstruction (UUO) Model of Rats
  • 10:56Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol using 2-photon microscopy in Munich Wistar Fromter rats with surface glomeruli to quantifythe effects of prolonged ureteral obstruction on glomerular dynamics and function.

Tags

Keywords: 2-photon MicroscopyUnilateral Ureteral ObstructionKidney FunctionInflammationFibrosisCell-cell InteractionsSubcellular EventsUreteral ProcedureAnesthetizeRenal PedicleUreterSutureAbdominal IncisionKidney PositionMuscle LayerHemostats

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