Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver

Purification of recombinant protein from bacteria is a well-established method. However, several problems may occur when using these techniques. Having methods to extract protein from tissue is for sure appealing.

The main advantage with this method is that one may extract the protein in its physiological form including all mutual effects that may impact its folding and catalytic activity. This approach may be adapted to other proteins of interest and modified for other tissue. Purified protein from tissue may support the development of high-quality antibodies and pharmacological inhibitors.