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Isolering af mitokondrier fra museskeletmuskulatur til respirometriske assays
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Isolering af mitokondrier fra museskeletmuskulatur til respirometriske assays

Article DOI: 10.3791/63336-v • 08:11 min • February 10th, 2022
February 10th, 2022 •
1,2, 1, 1,2, 1,2, 1, 1,2
1Centro Andaluz de Biología del Desarrollo, CSIC-UPO-JA, Universidad Pablo de Olavide, 2CIBERER, Instituto de Salud Carlos III

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Her beskriver vi en detaljeret metode til mitokondrier isolering fra musens skeletmuskulatur og den efterfølgende analyse af åndedræt ved Oxygen Consumption Rate (OCR) ved hjælp af mikropladebaserede respirometriske assays. Denne rørledning kan anvendes til at studere virkningerne af flere miljømæssige eller genetiske indgreb på mitokondriemetabolisme.

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