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Quantifying Bacterial Surface Swarming Motility on Inducer Gradient Plates
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Biochemistry
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JoVE Journal Biochemistry
Quantifying Bacterial Surface Swarming Motility on Inducer Gradient Plates

Quantifying Bacterial Surface Swarming Motility on Inducer Gradient Plates

DOI:
10.3791/63382-v

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05:57 min

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January 05, 2022

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, , , ,
1Department of Biomedical Engineering,Southern University of Science and Technology

Chapters

  • 00:05Introduction
  • 01:03Preparation of Double-Layer Gradient Swarm Plates
  • 01:51Inoculation and Incubation of Gradient Swarm Plates
  • 02:42Imaging Bacterial Surface Swarming
  • 03:29Quantify the Swarm Area Using ImageJ Software
  • 04:22Results: Quantification of Bacterial Surface Swarming Motility
  • 05:22Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe the use of inducer gradient plates to evaluate bacterial swarming motility while simultaneously obtaining multiple concentration responses.

Tags

Keywords: Bacterial SwarmingInducer GradientHigh-throughput ScreeningChemotaxisMicropathogen ResponseBiochemical CuesArabinoseSemisolid AgarCylindrical WellSwarm PlateGel Imaging System

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