Journal
/
Neuroscience
/
A Gravity-Fed Transcardial Perfusion Method for Histologic Analysis of the Mouse Central Nervous System
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
A Gravity-Fed Transcardial Perfusion Method for Histologic Analysis of the Mouse Central Nervous System

A Gravity-Fed Transcardial Perfusion Method for Histologic Analysis of the Mouse Central Nervous System

DOI:
10.3791/63386-v

•

10:37 min

•

January 21, 2022

•

, ,
1Department of Biochemistry and Molecular Biology,State University of New York Upstate Medical University, 2Department of Neurology,State University of New York Upstate Medical University, 3Department of Neuroscience and Physiology,State University of New York Upstate Medical University

Chapters

  • 00:04Introduction
  • 00:46Construction of Perfusion Apparatus and Dissection Platform
  • 02:40Preparation of the Paraformaldehyde Solution
  • 04:58Transcardial “Pump-Free” Perfusion
  • 09:18Results: Histological Analysis Following Gravity-Fed Perfusion
  • 10:09Conclusion

Summary

Automatic Translation

Automatic Translation

A convenient gravity-fed perfusion method for histological analysis of the mouse central nervous system is presented. Immunofluorescent detection of phosphorylated α-synuclein is demonstrated in a mouse model of Parkinson's disease. This work also comprehensively describes the transcardial perfusion, dissection, tissue freezing/embedding, and frozen sectioning steps.

Tags

Keywords: Gravity-fed Transcardial PerfusionHistologic AnalysisMouse Central Nervous SystemFixative PerfusionProtocolApparatusBuffer BottlesMicro SpatulasTubingSyringePBSPFAStyrofoam Block

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63386?article_type=v

Systemic and Local Drug Delivery for Treating Diseases of the Central Nervous System in Rodent Models
check_url/63386?article_type=v

Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System
check_url/63386?article_type=v

Multimodal Imaging of Stem Cell Implantation in the Central Nervous System of Mice
check_url/63386?article_type=v

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System
check_url/63386?article_type=v

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster
check_url/63386?article_type=v

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System
check_url/63386?article_type=v

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
check_url/63386?article_type=v

In vivo Optogenetic Stimulation of the Rodent Central Nervous System
check_url/63386?article_type=v

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY
check_url/63386?article_type=v

Electrophysiological Recording of The Central Nervous System Activity of Third-Instar Drosophila Melanogaster

Read Article