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Journal / Neuroscience / A Gravity-Fed Transcardial Perfusion Method for…
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Neuroscience

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JoVE Journal Neuroscience
A Gravity-Fed Transcardial Perfusion Method for Histologic Analysis of the Mouse Central Nervous System
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A Gravity-Fed Transcardial Perfusion Method for Histologic Analysis of the Mouse Central Nervous System

Article DOI: 10.3791/63386-v • 10:37 min • January 21st, 2022
January 21st, 2022 •
1, 2, 1,3
1Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, 2Department of Neurology, State University of New York Upstate Medical University, 3Department of Neuroscience and Physiology, State University of New York Upstate Medical University

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Summary
Automatic Translation

A convenient gravity-fed perfusion method for histological analysis of the mouse central nervous system is presented. Immunofluorescent detection of phosphorylated α-synuclein is demonstrated in a mouse model of Parkinson's disease. This work also comprehensively describes the transcardial perfusion, dissection, tissue freezing/embedding, and frozen sectioning steps.

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Gravity-fed Transcardial Perfusion Histologic Analysis Mouse Central Nervous System Fixative Perfusion CNS Tissues Gravity Perfusion Method Profusion Apparatus Easily Available Components Arnav Rana MD-PhD Student Xin Jie Chen's Laboratory Razor Blade Inner Straws Wash Bottles Buffer Bottles Stainless Steel Micro Spatula S-shaped Bend Hooks 25-centimeter-long Tubes Y Connector 2-meter-long Tubing 1-milliliter Syringe
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