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Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
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Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

DOI:
10.3791/63396-v

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10:33 min

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May 27, 2022

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, ,
1Department of Biology,McMaster University

Chapters

  • 00:04Introduction
  • 00:50Incubation of Soil in Liquid Broth and Transferring the Supernatant to the Solid Medium
  • 02:34Detection of Yeasts, Streaking for Single Colonies, and Identification of Yeast Species
  • 04:04Isolation of Aspergillus fumigatus from Soil
  • 07:32Results: Isolation and Characterization of Soil Yeast and Aspergillus fumigatus Populations
  • 09:37Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol is an effective, speedy method of culturing yeasts and the mold Aspergillus fumigatus from large sets of soil samples in as little as 7 days. The methods can be easily modified to accommodate a range of incubation media and temperatures as needed for experiments.

Tags

Keywords: Soil SamplesYeast DiversityMold DiversityFungal PopulationCulturable YeastsCulturable MoldsChloramphenicolBenomylYeast Extract-peptone-dextrose AgarRoller Drum IncubationBiosafety CabinetSoil Particle SuspensionColony SelectionStreak Plating

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