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JoVE Journal Bioengineering
体内 使用双光子荧光和受激拉曼散射显微镜对生物组织进行成像
Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation
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体内 使用双光子荧光和受激拉曼散射显微镜对生物组织进行成像

Article DOI: 10.3791/63411-v • 09:06 min • December 20th, 2021
December 20th, 2021 •
1, 1, 1,2,3,4, 5
1Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology, 2State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, 3Center of Systems Biology and Human Health, The Hong Kong University of Science and Technology, 4Molecular Neuroscience Center, The Hong Kong University of Science and Technology, 5Department of Biology, School of Life Sciences, Southern University of Science and Technology

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受激拉曼散射（SRS）显微镜允许根据特定化学键的固有振动对生物分子进行无标记成像。在该协议中，描述了集成SRS和双光子荧光显微镜的仪器设置，以可视化活小鼠脊髓中的细胞结构。

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生物工程，第178期，
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