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JoVE Journal Bioengineering
Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting
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Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting

Article DOI: 10.3791/63430-v • 07:40 min • March 31st, 2022
March 31st, 2022 •
1,2,3, 1,2,4, 1,2,4, 1,2, 2,3,4,5, 1,2,3,4
1Nucleus of Multidisciplinary Research in Biology (Numpex-Bio), Federal University of Rio de Janeiro, 2Laboratory of Tissue Bioengineering, National Institute of Metrology, Quality and Technology (Inmetro), 3Post-graduation Program of Translational Biomedicine (Biotrans), Unigranrio, 4Post-graduation Program in Biotechnology, National Institute of Metrology, Quality and Technology (Inmetro), 5Dental School, Fluminense Federal Fluminense

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Here, we describe the large-scale production of adipose-derived stromal/stem cell (ASC) spheroids using an automated pipetting system to seed the cell suspension, thus ensuring homogeneity of spheroid size and shape. These ASC spheroids can be used as building blocks for 3D bioprinting approaches.

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Large-Scale Automated Production Adipose-Derived Stem Cell Spheroids 3D Bioprinting Human Errors Microbiology Contamination Labor Hours Software Parameters Cell Culture Media Visual Demonstration Stem Cell Suspension Centrifuge Tube ASC Pellet DMEM-Low FBS Cell Count Recessions Micromolded Non-adherent Hydrogel Sterile 2% Ultra Pure Agarose Solution Silicone Mold Circular Recesses Plastic Plate
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