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Producción automatizada a gran escala de esferoides de células madre derivadas de tejido adiposo para bioimpresión 3D
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1Nucleus of Multidisciplinary Research in Biology (Numpex-Bio),Federal University of Rio de Janeiro, 2Laboratory of Tissue Bioengineering,National Institute of Metrology, Quality and Technology (Inmetro), 3Post-graduation Program of Translational Biomedicine (Biotrans),Unigranrio, 4Post-graduation Program in Biotechnology,National Institute of Metrology, Quality and Technology (Inmetro), 5Dental School,Fluminense Federal Fluminense
Chapters
- 00:05Introduction
- 00:55Trypsinization of ASC Monolayer at Passage Three
- 01:45Micro Molded Non-Adherent Agarose Hydrogel Fabrication
- 02:31Bio-Fabrication of ASC Spheroids Using the Automated Pipetting System
- 05:48Results: Automated Pipette System for Large-Scale Generation of ASC Spheroids
- 07:17Conclusion
Aquí, describimos la producción a gran escala de esferoides estromales /células madre (ASC) derivados de tejido adiposo utilizando un sistema de pipeteo automatizado para sembrar la suspensión celular, asegurando así la homogeneidad del tamaño y la forma del esferoide. Estos esferoides ASC se pueden utilizar como bloques de construcción para enfoques de bioimpresión 3D.
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3D BioprintingAdipose-derived Stem CellsSpheroidsAutomated ProductionMicrobiology ContaminationLabor HoursSoftware ParametersCell Culture Media3D Cell CultureStem Cell SuspensionASC PelletDMEM-LowFBSMicromolded AgaroseUltra Pure AgaroseSilicone Mold12-well Plastic PlateLaminar FlowCarbon DioxideLabWare EditorVirtual Work TablePipettesTip BoxesPlastic TubesPlates
