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Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin
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JoVE Journal Neuroscience
Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin

Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin

DOI:
10.3791/63449-v

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08:07 min

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March 24, 2023

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, , , ,
1Centro de Investigaciones en Óptica A.C. (CIO), 2Division of Sciences and Engineering, Department of Chemical, Electronic, and Biomedical Engineering,Universidad de Guanajuato, 3Institute of Physiology,Benemérita Universidad Autónoma de Puebla, 4Dirección General de Desarrollo Internacional,Benemérita Universidad Autónoma de Puebla

Chapters

  • 00:04Introduction
  • 01:02Microscope Settings
  • 02:00Microscope Preliminary Controls
  • 03:00Vibratome Sectioning
  • 04:00Transfer to the Microscope and Imaging
  • 05:15Processing of the Cerebellum
  • 05:35Results: Visualization of Tubulin-Dependent Defects in Central Myelin
  • 06:38Conclusion

Summary

Automatic Translation

Automatic Translation

In this article, we present a protocol to detect microtubule-loaded oligodendrocytes in a model of tubulinopathy through a simple, innovative second harmonic generation microscopy approach.

Tags

Keywords: SHG ImagingLabel-freeNon-linear OpticsTubulinCentral MyelinTubulinopathiesMicrotubulesLaser PolarizationVibratomeHBSS

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