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Slicing the Embryonic Chicken Auditory Brainstem to Evaluate Tonotopic Gradients and Microcircuits
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Chapters
- 00:04Introduction
- 00:47Egg Handling and Preparing ACSF and Agarose Block
- 01:52Isolating Auditory Brainstem
- 03:53Slicing and Handling Fragile Pieces of Brainstem Tissue
- 05:59Results: Evaluation of Tonotopic Gradients in the Brainstem
- 07:47Conclusion
Here we present a protocol for obtaining non-coronal auditory brainstem slices of the chicken embryo for the investigation of tonotopic properties and developmental trajectories within one brainstem slice. These slices include sagittal, horizontal, and horizontal/transverse sections encompassing larger tonotopic regions within an individual slice plane that the traditional coronal sections.
