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JoVE Journal Bioengineering
Realtids, tofarvet stimuleret Raman-spredningsbilleddannelse af musehjerne til vævsdiagnose
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Realtids, tofarvet stimuleret Raman-spredningsbilleddannelse af musehjerne til vævsdiagnose

Article DOI: 10.3791/63484-v • 10:57 min • February 1st, 2022
February 1st, 2022 •
*1, *1, 1
1Department of Chemistry, University of Washington
* These authors contributed equally

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Stimuleret Raman scattering (SRS) mikroskopi er en kraftfuld, ikke-destruktiv og etiketfri billeddannelsesteknik. En ny applikation er stimuleret Raman histologi, hvor tofarvet SRS-billeddannelse ved protein- og lipid Raman-overgangene bruges til at generere pseudo-hæmatoxylin- og eosinbilleder. Her demonstrerer vi en protokol til realtids, tofarvet SRS-billeddannelse til vævsdiagnose.

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