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JoVE Journal Bioengineering
Sanntids, tofarget stimulert ramanspredningsavbildning av musehjerne for vevsdiagnose
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High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy
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Sanntids, tofarget stimulert ramanspredningsavbildning av musehjerne for vevsdiagnose

Article DOI: 10.3791/63484-v • 10:57 min • February 1st, 2022
February 1st, 2022 •
*1, *1, 1
1Department of Chemistry, University of Washington
* These authors contributed equally

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Stimulert Raman-spredning (SRS) mikroskopi er en kraftig, ikke-ødeleggende og etikettfri bildeteknikk. En fremvoksende applikasjon stimuleres Raman histologi, hvor tofarget SRS-avbildning ved protein- og lipid Raman-overgangene brukes til å generere pseudo-hematoksylin- og eosinbilder. Her demonstrerer vi en protokoll for sanntids, tofarget SRS-avbildning for vevsdiagnose.

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