JoVE Journal
Genetics
Genetics
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基于半导体的下一代测序平台上非整倍体的植入前基因检测
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Article
DOI:
10.3791/63493-v
•
09:30 min
•
August 17th, 2022
基于半导体的下一代测序平台上非整倍体的植入前基因检测
August 17th, 2022
•
Chengming Xu*1, Riqing Wei*2, Hui Lin1, Leiyu Deng1, Li Wang3, Deyang Li4, Honghui Den5, Wensong Qin1, Ping Wen1, Ying Liu1, Yingsong Wu2, Qiang Ma2, Jinliang Duan1
1Centre for Women, Children, and Reproduction, Guangxi Key Laboratory of Metabolic Diseases Research, 2Department of Biopharmaceutics, School of Laboratory Medicine and Biotechnology, Southern Medical University, 3Department of Obstetrics and Gynecology, Chinese PLA General Hospital, 4Clinical Laboratory, Northern Theater Air Force Hospital, 5Guangzhou Darui Reproduction Technology Co., Ltd.
* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
该协议介绍了在基于半导体的下一代测序平台上对非整倍性进行植入前基因检测所需的整体实验室内程序。在这里,我们以具有代表性的结果介绍了全基因组扩增、DNA片段选择、文库构建、模板制备和测序工作流程的详细步骤。