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Journal / Developmental Biology / Augenentfernung in lebenden Zebrafischlarven zur…
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JoVE Journal Developmental Biology
Augenentfernung in lebenden Zebrafischlarven zur Untersuchung des innervationsabhängigen Wachstums und der Entwicklung des visuellen Systems
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Augenentfernung in lebenden Zebrafischlarven zur Untersuchung des innervationsabhängigen Wachstums und der Entwicklung des visuellen Systems

Article DOI: 10.3791/63509-v • 09:00 min • February 11th, 2022
February 11th, 2022 •
1, 1, 1,2, 3, 1
1Department of Biology, Reed College, 2Committee on Development, Regeneration, and Stem Cell Biology, University of Chicago, 3Department of Cell and Developmental Biology, University College London

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Der Artikel erklärt, wie man Augen von lebenden Zebrafischlarven chirurgisch entfernt, als ersten Schritt zur Untersuchung, wie der retinale Input das Wachstum und die Entwicklung des optischen Tektums beeinflusst. Darüber hinaus enthält der Artikel Informationen über Larvenanästhesie, Fixierung und Hirndissektion, gefolgt von Immunhistochemie und konfokaler Bildgebung.

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Entwicklungsbiologie Ausgabe 180 Zebrafisch Visuelles System Optic tectum Augenexstirpation Hirndissektion Immunhistochemie Proliferation Axonale Degeneration
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