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Isolierung von primären retinalen pigmentierten Epithelzellen der Maus
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Isolierung von primären retinalen pigmentierten Epithelzellen der Maus

Article DOI: 10.3791/63543-v • 06:21 min • November 4th, 2022
November 4th, 2022 •
1,2, 3, 1,2, 1,2,3
1Eye Research Institute, Oakland University, 2Eye Research Center (OUWB)/ERC, William Beaumont School of Medicine, 3Oral Biology and Diagnostic Sciences, Dental College of Georgia, Augusta University

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Dieses Manuskript beschreibt ein vereinfachtes Protokoll zur schrittweisen Isolierung von retinalen pigmentierten Epithelzellen (RPE) aus Mausaugen. Das Protokoll umfasst die Enukleation und Dissektion von Mausaugen, gefolgt von der Isolierung, Aussaat und Kultivierung von RPE-Zellen.

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Biologie Ausgabe 189
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