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Journal / Biology / Primer Fare Retinal Pigmentli Epitel Hücrelerinin …
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Primer Fare Retinal Pigmentli Epitel Hücrelerinin İzolasyonu
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Primer Fare Retinal Pigmentli Epitel Hücrelerinin İzolasyonu

Article DOI: 10.3791/63543-v • 06:21 min • November 4th, 2022
November 4th, 2022 •
1,2, 3, 1,2, 1,2,3
1Eye Research Institute, Oakland University, 2Eye Research Center (OUWB)/ERC, William Beaumont School of Medicine, 3Oral Biology and Diagnostic Sciences, Dental College of Georgia, Augusta University

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Bu yazıda retinal pigmente epitel (RPE) hücrelerinin fare gözlerinden kademeli olarak izole edilmesi için basitleştirilmiş bir protokol anlatılmaktadır. Protokol, fare gözlerinin enükleasyonunu ve diseksiyonunu, ardından RPE hücrelerinin izolasyonunu, tohumlanmasını ve kültürlenmesini içerir.

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