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Uma abordagem de ensaio de cometa de alto rendimento para avaliar danos ao DNA celular
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Uma abordagem de ensaio de cometa de alto rendimento para avaliar danos ao DNA celular

Article DOI: 10.3791/63559-v • 07:57 min • May 10th, 2022
May 10th, 2022 •
*1, *2, *3, 4, 5, 1
1Oxidative Stress Group, Dept. Cell Biology, Microbiology and Molecular Biology, College of Arts and Sciences, University of South Florida, 2Cepheid (Danaher Corp.), US Technical Operations, 3Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, 4Department of Chemistry and Biochemistry, Institute of Environment, Florida International University, 5Leicester School of Allied Health Sciences, Faculty of Health and Life Sciences, De Montfort University
* These authors contributed equally

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O ensaio de cometa é um meio popular de detectar danos ao DNA. Este estudo descreve uma abordagem para a execução de lâminas em variantes representativas do ensaio do cometa. Essa abordagem aumentou significativamente o número de amostras, diminuindo o tempo de execução do ensaio, o número de manipulações de lâminas e o risco de danos aos géis.

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Biologia Edição 183 Ensaio de cometa Danos ao DNA Reparo do DNA estresse oxidativo biomarcador eletroforese
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