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Assessing Energy Substrate Oxidation In Vitro with ¹⁴CO₂ Trapping

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JoVE Journal Biology

Assessing Energy Substrate Oxidation In Vitro with ¹⁴CO₂ Trapping

Assessing Energy Substrate Oxidation In Vitro with ¹⁴CO₂ Trapping

DOI:

10.3791/63568-v

•

09:20 min

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March 23, 2022

•

Chao Song², Arianna Valeri, Fanxin Long

¹Translational Research Program of Pediatric Orthopedics, Department of Surgery,The Children’s Hospital of Philadelphia, ²Department of Orthopedic Surgery, Tongji Hospital,Huazhong University of Science and Technology

Chapters

00:04Introduction
00:59Isolation and Culture of Calvarial Preosteoblasts
03:39Isolation and Culture of Bone Marrow-Derived Macrophages
05:06Substrate Oxidation Assay with Carbon Dioxide Trap
07:39Results: Substrate Oxidation Rates in Calvarial Preosteoblasts Versus BMMs
08:33Conclusion

Summary

Automatic Translation

This protocol describes an easy-to-use method to examine substrate oxidation by tracking ¹⁴CO₂production in vitro.

Tags

Keywords: 14CO2 Trapping Energy Substrate Oxidation Metabolic Flexibility Cell Type-specific Metabolic Disorders Dietary And Pharmacological Interventions Cell Culture Collagenase Digestion MEM Alpha Medium Trypsin-EDTA Cell Counting Substrate Oxidation Rate

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