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Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue
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Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue

Article DOI: 10.3791/63606-v • 08:12 min • May 5th, 2022
May 5th, 2022 •
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1Department of Biochemistry, Albert Einstein College of Medicine

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This protocol outlines how a three-dimensional cell culture system can be used to model, treat, and analyze chromatin modifications in a near-physiological state.

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Global Quantification Histone Post-translational Modifications 3D Cell Culture Model Hepatic Tissue Chromatin Dynamics Disease States Cancer Aging Protocol Physiologically Relevant Model Laboratory Analysis Histone PTMs Antibody-based Assays Liquid Chromatography Mass Spectrometry Analysis Abundance Experiment Stephanie Stransky Ronald Cutler Julie Kim Postdoc Grad Student Research Tech Growth Media Monolayer Confluent Wash Cells HBSS Trypsin-EDTA Incubate Cells Carbon Dioxide Concentration Microscope Cell Detachment Counting Cells Cell Suspension Dilution Ultra Low Attachment 24-well Round Bottom Plate Centrifugation
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