Name: Mouse Abdominal Aortic Aneurysm Model Induced by Perivascular Application of Elastase (Video) | JoVE Journal
Uploaded: 2022-02-11T15:00:00.000+00:00
Duration: 4 min 49 s
Description: Learn a standardized surgical protocol for inducing abdominal aortic aneurysm in mice using perivascular elastase application. This method achieves high reproducibility with 91.7% incidence in females and low operational costs.

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We thank the Unit for Laboratory Animal Medicine of the University of Michigan for their help with animal feeding and breeding. This study is supported by NIH RO1 HL138139, NIH RO1 HL153710 to&#160;J. Zhang, NIH RO1 HL109946, RO1 HL134569 to Y.E. Chen, and the American Heart Association grant 20POST35110064 to G. Zhao.

The animal protocols were approved by the University of Michigan Institutional Animal Care and Use Committee (PRO00010092). Male and female C57BL/6J wild-type (WT) mice, ~7 weeks of age, were used for the experiments.
1. Animal preparation
<ol>
	<li>Feed the mice with the standard chow diet (see Table of Materials) before and after the surgery. 
	&#8203;NOTE: Different strains and ages of mice can be used. However, age ranging from 5.5-12 week-old is recommended to achieve maximal incidence rate.</li>
	<li>For each mouse, administer 5 mg/kg of Carprofen subcutaneously 30 min before the induction of anesthesia.</li>
	<li>After 30 min, administer 100 mg/kg of Ketamine and 5mg/kg of Xylazine via intraperitoneal injection to induce anesthesia.</li>
</ol>
2. Preparation for surgery
<ol>
	<li>Prepare the surgical material.
	<ol>
		<li>Cut the nitrile gloves into strips of 4 cm x 4 mm. Cut the cotton pads into pieces of 3 cm x 2 mm. Autoclave these with other surgical instruments, including surgical scissors, tissue forceps, and Halsted-Mosquito hemostats (see Table of Materials).</li>
	</ol>
	</li>
	<li>Place the mouse in the supine position on a sterile absorbent dressing pad. Immobilize the front and hind paws with surgical tape.</li>
	<li>Use cotton-tipped applicators (see Table of Materials) to brush hair remover lotion over the middle and lower abdominal area, then swab the area with surgical gauze to remove the hair.</li>
	<li>Disinfect the surgical area&#160;at least three times in a circular motion with alternating applications of 70% alcohol and an iodine-based or chlorhexidine-based scrub. Allow to dry.</li>
</ol>
3. Surgery procedure
<ol>
	<li>Perform the following steps to access the abdominal cavity.
	<ol>
		<li>Test the mouse for the lack of toe-pinch response before skin incision.</li>
		<li>Make a 2.5 cm longitudinal incision on the skin along the midline of the middle and lower abdomen using surgical scissors.</li>
		<li>Gently pull up the underlying muscle and make a 2.5 cm longitudinal incision along linea alba to access the abdominal cavity.</li>
	</ol>
	</li>
	<li>Expose the abdominal aorta.
	<ol>
		<li>Use wetted cotton-tipped applicators to move the intestines and stomach to the right side of the mouse. 
		NOTE: Ideally, this will expose the infrarenal segment of the aorta. If the aorta is hard to locate, the right kidney and right renal artery can identify the aorta (as the right kidney has a slightly lower anatomical location than the left kidney).</li>
		<li>Use forceps to gently remove the connective tissue covering the abdominal aorta and inferior vena cava (IVC). 
		NOTE: The abdominal aorta and IVC are contained within the same vessel sheath. It is unnecessary to remove all the connective tissue as complete removal would increase the risk of damaging these two vessels.</li>
		<li>Use forceps to gently dissect the backside of the abdominal aorta and IVC from the underlying muscles. 
		NOTE: Tips of the forceps should go into the backside of the sheath transversely and create a hole in the fascia that connects the sheath to the underlying muscles. Once the hole is made, extend its size by slowly releasing the forceps.</li>
		<li>Place a piece of the 4 cm x 4 mm glove stripe (as mentioned earlier, step 2.1.1) through the backside of the abdominal aorta and IVC, then straighten the stripe. Place the stripe ~0.5 cm away from the right renal artery. 
		NOTE: Ensure that the hole is big enough so that the surrounding fascia will not twist the stripe.</li>
		<li>Above the stripe, place a piece of 3 cm x 2 mm cotton pad through the backside of the abdominal aorta and IVC, then straighten the cotton pad.</li>
	</ol>
	</li>
	<li>Incubate the elastase.
	<ol>
		<li>Use a pipette to drop 30 &#181;L of porcine pancreatic elastase (total enzymatic activity of 1.8 unit, see Table of Materials) onto the aorta segment above the cotton pad, then wrap the cotton pad and stripe around the aorta and IVC. Rinse a piece of 10 cm x 10 cm gauze with sterile 0.9% saline and place it on the abdomen. 
		&#8203;NOTE: The gauze needs only partial rinsing as overdoing would risk diluting the elastase beneath.</li>
		<li>After 30 min, remove the stripe and cotton pad with forceps.</li>
	</ol>
	</li>
	<li>Close the abdominal cavity following the steps below.
	<ol>
		<li>Irrigate the aorta and abdominal cavity with 500 &#181;L of sterile 0.9% saline. Use a 10 cm x 10 cm gauze to absorb the remaining saline.</li>
		<li>Reapproximate the muscle layers with a running 6-0 non-absorbable monofilament suture.</li>
		<li>Close skin with 3-4 interrupted 6-0 non-absorbable monofilament sutures.</li>
	</ol>
	</li>
</ol>
4. Post-operative care
<ol>
	<li>Administer 5mg/kg of Carprofen subcutaneously on the post-operative Day 1.</li>
	<li>Remove skin sutures on the post-operative Day 10.</li>
</ol>
5. Measurement of abdominal aorta aneurysm diameter
<ol>
	<li>Euthanize the mice by CO2 overdosing on post-operative Day 14. This represents the time point of maximal dilatation.</li>
	<li>Access the abdominal cavity as described in step 3.1.</li>
	<li>Perform vascular perfusion by injecting 10 mL of 0.9% saline into the circulation through the left ventricle.</li>
	<li>Expose the infrarenal segment of the abdominal aorta as described in steps 3.1-3.2. Carefully remove the surrounding connective tissue and separate the abdominal aorta from IVC.</li>
	<li>Measure the diameter of the abdominal aorta with a caliper.</li>
</ol>

<ol><li>Kent, K. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Clinical+practice%3A+Abdominal+aortic+aneurysms%5BTitle%5D)+AND+%22The+New+England+Journal+of+Medicine%22%5BJournal%5D)">Clinical practice: Abdominal aortic aneurysms.</a> The New England Journal of Medicine. 371 (22), 2101-2108 (2014).</li>
<li>Karthikesalingam, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mortality+from+ruptured+abdominal+aortic+aneurysms%3A+Clinical+lessons+from+a+comparison+of+outcomes+in+England+and+the+USA%5BTitle%5D)+AND+%22Lancet%22%5BJournal%5D)">Mortality from ruptured abdominal aortic aneurysms: Clinical lessons from a comparison of outcomes in England and the USA.</a> Lancet. 383 (9921), 963-969 (2014).</li>
<li>Noel, A. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Ruptured+abdominal+aortic+aneurysms%3A+The+excessive+mortality+rate+of+conventional+repair%5BTitle%5D)+AND+%22Journal+of+Vascular+Surgery%22%5BJournal%5D)">Ruptured abdominal aortic aneurysms: The excessive mortality rate of conventional repair.</a> Journal of Vascular Surgery. 34 (1), 41-46 (2001).</li>
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<li>Kochanek, K. D., Xu, J., Murphy, S. L., Minino, A. M., Kung, H. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Deaths%3A+Final+data+for+2009%5BTitle%5D)+AND+%22National+Vital+Statistics+Reports%22%5BJournal%5D)">Deaths: Final data for 2009.</a> National Vital Statistics Reports. 60 (3), 1(2011).</li>
<li>Daugherty, A., Cassis, L. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mechanisms+of+abdominal+aortic+aneurysm+formation%5BTitle%5D)+AND+%22Current+Atherosclerosis+Reports%22%5BJournal%5D)">Mechanisms of abdominal aortic aneurysm formation.</a> Current Atherosclerosis Reports. 4 (3), 222-227 (2002).</li>
<li>Quintana, R. A., Taylor, W. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cellular+mechanisms+of+aortic+aneurysm+formation%5BTitle%5D)+AND+%22Circulation+Research%22%5BJournal%5D)">Cellular mechanisms of aortic aneurysm formation.</a> Circulation Research. 124 (4), 607-618 (2019).</li>
<li>Kuivaniemi, H., Ryer, E. J., Elmore, J. R., Tromp, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Understanding+the+pathogenesis+of+abdominal+aortic+aneurysms%5BTitle%5D)+AND+%22Expert+Review+of+Cardiovascular+Therapy%22%5BJournal%5D)">Understanding the pathogenesis of abdominal aortic aneurysms.</a> Expert Review of Cardiovascular Therapy. 13 (9), 975-987 (2015).</li>
<li>Trollope, A., Moxon, J. V., Moran, C. S., Golledge, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Animal+models+of+abdominal+aortic+aneurysm+and+their+role+in+furthering+management+of+human+disease%5BTitle%5D)+AND+%22Cardiovascular+Pathology%22%5BJournal%5D)">Animal models of abdominal aortic aneurysm and their role in furthering management of human disease.</a> Cardiovascular Pathology. 20 (2), 114-123 (2011).</li>
<li>Patelis, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Animal+models+in+the+research+of+abdominal+aortic+aneurysms+development%5BTitle%5D)+AND+%22Physiological+Research%22%5BJournal%5D)">Animal models in the research of abdominal aortic aneurysms development.</a> Physiological Research. 66 (6), 899-915 (2017).</li>
<li>Senemaud, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Translational+relevance+and+recent+advances+of+animal+models+of+abdominal+aortic+aneurysm%5BTitle%5D)+AND+%22Arteriosclerosis%2C+Thrombosis%2C+and+Vascular+Biology%22%5BJournal%5D)">Translational relevance and recent advances of animal models of abdominal aortic aneurysm.</a> Arteriosclerosis, Thrombosis, and Vascular Biology. 37 (3), 401-410 (2017).</li>
<li>Lysgaard Poulsen, J., Stubbe, J., Lindholt, J. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Animal+models+used+to+explore+abdominal+aortic+aneurysms%3A+A+systematic+review%5BTitle%5D)+AND+%22European+Journal+of+Vascular+and+Endovascular+Surgery%22%5BJournal%5D)">Animal models used to explore abdominal aortic aneurysms: A systematic review.</a> European Journal of Vascular and Endovascular Surgery. 52 (4), 487-499 (2016).</li>
<li>Tsui, J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Experimental+models+of+abdominal+aortic+aneurysms%5BTitle%5D)+AND+%22Open+Cardiovascular+Medicine+Journal%22%5BJournal%5D)">Experimental models of abdominal aortic aneurysms.</a> Open Cardiovascular Medicine Journal. 4, 221-230 (2010).</li>
<li>Manning, M. W., Cassi, L. A., Huang, J., Szilvassy, S. J., Daugherty, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Abdominal+aortic+aneurysms%3A+Fresh+insights+from+a+novel+animal+model+of+the+disease%5BTitle%5D)+AND+%22Vascular+Medicine%22%5BJournal%5D)">Abdominal aortic aneurysms: Fresh insights from a novel animal model of the disease.</a> Vascular Medicine. 7 (1), 45-54 (2002).</li>
<li>Chiou, A. C., Chiu, B., Pearce, W. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Murine+aortic+aneurysm+produced+by+periarterial+application+of+calcium+chloride%5BTitle%5D)+AND+%22Journal+of+Surgical+Research%22%5BJournal%5D)">Murine aortic aneurysm produced by periarterial application of calcium chloride.</a> Journal of Surgical Research. 99 (2), 371-376 (2001).</li>
<li>Allaire, E., Guettier, C., Bruneval, P., Plissonnier, D., Michel, J. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cell-free+arterial+grafts%3A+Morphologic+characteristics+of+aortic+isografts%2C+allografts%2C+and+xenografts+in+rats%5BTitle%5D)+AND+%22Journal+of+Vascular+Surgery%22%5BJournal%5D)">Cell-free arterial grafts: Morphologic characteristics of aortic isografts, allografts, and xenografts in rats.</a> Journal of Vascular Surgery. 19 (3), 446-456 (1994).</li>
<li>Anidjar, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Elastase-induced+experimental+aneurysms+in+rats%5BTitle%5D)+AND+%22Circulation%22%5BJournal%5D)">Elastase-induced experimental aneurysms in rats.</a> Circulation. 82 (3), 973-981 (1990).</li>
<li>Sun, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mast+cells+modulate+the+pathogenesis+of+elastase-induced+abdominal+aortic+aneurysms+in+mice%5BTitle%5D)+AND+%22Journal+of+Clinical+Investigation%22%5BJournal%5D)">Mast cells modulate the pathogenesis of elastase-induced abdominal aortic aneurysms in mice.</a> Journal of Clinical Investigation. 117 (11), 3359-3368 (2007).</li>
<li>Bhamidipati, C. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Development+of+a+novel+murine+model+of+aortic+aneurysms+using+peri-adventitial+elastase%5BTitle%5D)+AND+%22Surgery%22%5BJournal%5D)">Development of a novel murine model of aortic aneurysms using peri-adventitial elastase.</a> Surgery. 152 (2), 238-246 (2012).</li>
<li>Pyo, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Targeted+gene+disruption+of+matrix+metalloproteinase-9+%28gelatinase+B%29+suppresses+development+of+experimental+abdominal+aortic+aneurysms%5BTitle%5D)+AND+%22Journal+of+Clinical+Investigation%22%5BJournal%5D)">Targeted gene disruption of matrix metalloproteinase-9 (gelatinase B) suppresses development of experimental abdominal aortic aneurysms.</a> Journal of Clinical Investigation. 105 (11), 1641-1649 (2000).</li>
<li>Tanaka, A., Hasegawa, T., Chen, Z., Okita, Y., Okada, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+novel+rat+model+of+abdominal+aortic+aneurysm+using+a+combination+of+intraluminal+elastase+infusion+and+extraluminal+calcium+chloride+exposure%5BTitle%5D)+AND+%22Journal+of+Vascular+Surgery%22%5BJournal%5D)">A novel rat model of abdominal aortic aneurysm using a combination of intraluminal elastase infusion and extraluminal calcium chloride exposure.</a> Journal of Vascular Surgery. 50 (6), 1423-1432 (2009).</li>
<li>Busch, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Four+surgical+modifications+to+the+classic+elastase+perfusion+aneurysm+model+enable+haemodynamic+alterations+and+extended+elastase+perfusion%5BTitle%5D)+AND+%22European+Journal+of+Vascular+and+Endovascular+Surgery%22%5BJournal%5D)">Four surgical modifications to the classic elastase perfusion aneurysm model enable haemodynamic alterations and extended elastase perfusion.</a> European Journal of Vascular and Endovascular Surgery. 56 (1), 102-109 (2018).</li>
<li>Liang, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((KLF11+protects+against+venous+thrombosis+via+suppressing+tissue+factor+expression%5BTitle%5D)+AND+%22Thrombosis+and+Haemostasis%22%5BJournal%5D)">KLF11 protects against venous thrombosis via suppressing tissue factor expression.</a> Thrombosis and Haemostasis. , (2021).</li>
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<li>Shannon, A. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Porcine+model+of+infrarenal+abdominal+aortic+aneurysm%5BTitle%5D)+AND+%22Journal+of+Visualized+Experiments%22%5BJournal%5D)">Porcine model of infrarenal abdominal aortic aneurysm.</a> Journal of Visualized Experiments. (153), e60169(2019).</li>
</ol>

The authors have nothing to disclose.

The elastase-induced AAA model was first reported by Anidjar et al. using rats in 199017. A variety of modified versions have been introduced in the past thirty years, along with significant improvement in the surgical techniques19,20,21,22. Hundreds of institutes use elastase-induced AAA models as the second most used rodent experimental model for AAA studies<sup class=...

Abdominal aortic aneurysm (AAA) is defined as a segmental dilatation of the abdominal aorta with at least a 50% increase of vessel diameter1. AAA is potentially fatal, as the rupture can result in an extremely high mortality rate, even with intervention2,3,4. It has been reported that AAA is responsible for approximately 13,000 deaths annually in the USA, which makes it the 10th leading cause of death1,5.
The pathogenesis of AAA is not yet wholly understood6,7,8. To investigate the molecular mechanism of AAA and test potential therapeutic targets, several experimental AAA models have been established9,10. Rodent models of AAA include elastase, calcium chloride, angiotensin II and xenograft models, among which the elastase-induced AAA model is the second most used model10,11,12,13,14,15,16,17. This model involves direct infusion or application of porcine pancreatic elastase (PPE) to the infrarenal segment of the aorta. The penetration of elastase into the medial layer of the aorta will cause degradation of elastic lamellae and infiltration of inflammatory cells, leading to the loss of aortic wall integrity and subsequent dilation of abdominal aorta7,18. The elastase-induced AAA model was first reported by Anidjar et al. in 1990 using rats, in which an isolated segment of the aorta was perfused with elastase17. Later in 2012, a modified model using a periadventitial application of PPE was reported by Bhamidipati et al.19. Nowadays, most surgeries for the elastase-induced AAA model are inspired by Bhamidipati&#39;s group and are performed with the external application rather than intraluminal perfusion of PPE. Although the external application has less requirement on fine surgical skills, the incidence rate of AAA is relatively lower and size somewhat smaller than that of intraluminal perfusion11,19.
Although widely used in AAA studies, the elastase-induced AAA model possesses certain limitations. One caveat of this model is the inevitable variations of how the surgery is performed, which can lead to the issue of reproducibility. For example, the difference may exist in the surgical procedure regarding how the infrarenal segment of the aorta is isolated and which part of the segment is selected for PPE application among different laboratories. The enzymatic activity of PPE and the time duration of PPE incubation can also vary. These, however, are all essential determinants that affect the eventual AAA formation rate and aneurysm diameter.The variation of these critical determinants makes data comparison of AAA studies from different groups using this model very difficult. Therefore, a standardized surgical procedure is needed as a tool to get comparable results from various institutions.
This article describes a standardized surgical protocol for the elastase-induced AAA model through direct application of PPE to the adventitia of infrarenal abdominal aorta in mice. Details about surgical material and procedures essential for successful and robust generation of AAA in mice using this model will also be discussed.

<table><tbody><tr><td>6-0 non-absorbable monofilament suture</td><td>Pro Advantage</td><td>P420697</td><td></td></tr><tr><td>Carprofen</td><td>Zoetis Inc.</td><td>NDC: 54771-8507</td><td></td></tr><tr><td>Chow Diet</td><td>LabDiet</td><td>3005659-220</td><td>PicoLab 5L0D</td></tr><tr><td>Cotton Applicator</td><td>Dynarex</td><td>4303</td><td></td></tr><tr><td>Cotton Pad</td><td>Rael</td><td>UPC: 810027130969</td><td></td></tr><tr><td>GraphPad Prism 8</td><td>GraphPad Software Inc.</td><td></td><td>Version 8.4.3</td></tr><tr><td>Grarfe Forceps</td><td>Fine Science Tools</td><td>11051-10</td><td></td></tr><tr><td>Halsted Mosquito Hemostats</td><td>Fine Science Tools</td><td>13009-12</td><td></td></tr><tr><td>Ketamine</td><td>Par Pharmaceutical</td><td>NDC: 42023-0115-10</td><td></td></tr><tr><td>Nitrile gloves</td><td>Fisherbrand</td><td>19-130-1597</td><td></td></tr><tr><td>Penicillin-Streptomycin</td><td>Thermo Fisher</td><td>15140122</td><td></td></tr><tr><td>Porcine pancreatic elastase</td><td>Sigma-Aldrich</td><td>E1250-100MG</td><td></td></tr><tr><td>Scissors</td><td>Fine Science Tools</td><td>14068-12</td><td></td></tr><tr><td>Sterile 0.9% saline solution</td><td>Baxter</td><td>2B1324X</td><td></td></tr><tr><td>Xylazine</td><td>Akorn</td><td>NDC: 59399-110-20</td><td></td></tr></tbody></table>

mouse abdominal aortic aneurysm model induced by perivascular application of elastase

Mouse Abdominal Aortic Aneurysm Model Induced by Perivascular Application of Elastase

Abdominal aortic aneurysm (AAA), although primarily asymptomatic, is potentially life-threatening as the rupture of AAA usually has a devastating outcome. Currently, there are several distinct experimental models of AAA, each emphasizing a different aspect in the pathogenesis of AAA. The elastase-induced AAA model is the second most used rodent AAA model. This model involves direct infusion or application of porcine pancreatic elastase (PPE) to the infrarenal segment of the aorta. Due to technical challenges, most elastase-induced AAA model nowadays is performed with the external application rather than an intraluminal infusion of PPE. The infiltration of elastase will cause degradation of elastic lamellae in the medial layers, resulting in the loss of aortic wall integrity and subsequent dilation of the abdominal aorta. However, one disadvantage of the elastase-induced AAA model is the inevitable variation of how the surgery is performed. Specifically, the surgical technique of isolating the infrarenal segment of the aorta, the material used for aorta wrapping and PPE incubation, the enzymatic activity of PPE, and the time duration of PPE application can all be important determinants that affect the eventual AAA formation rate and aneurysm diameter. Notably, the difference in these factors from different studies on AAA can lead to reproducibility issues. This article describes a detailed surgical process of the elastase-induced AAA model through direct application of PPE to the adventitia of the infrarenal abdominal aorta in the mouse. Following this procedure, a stable AAA formation rate of around 80% in male and female mice is achievable. The consistency and reproducibility of AAA studies using an elastase-induced AAA model can be significantly enhanced by establishing a standard surgical procedure.

A total of twenty-three 7-week-old wild-type (WT) mice, including 12 females and 11 males, were operated following the presented protocol. The survival rate was 100% (surgical mortality excluded). Maximal abdominal aorta diameter was measured by a caliper.
AAA was defined as dilating the abdominal aorta with a 50% vessel diameter increase. Therefore, a 50% increase in the maximal abdominal aorta diameter was selected as the cut-off point for successful AAA induction. Based upon this criterion,...

The present protocol describes a standardized surgical method for the elastase-induced AAA model through the direct application of elastase to the adventitia of infrarenal abdominal aorta in mice.

Abdominal aortic aneurysm (AAA), although primarily asymptomatic, is potentially life-threatening as the rupture of AAA usually has a devastating outcome. ...

mouse-abdominal-aortic-aneurysm-model-induced-perivascular

Research

JoVE Journal

Biology

This video shows how to induce abdominal aortic aneurysms (AAA) in mice via transient intraluminal infusion of porcine pancreatic elastase into the infrarenal segment of the abdominal aorta. The model has the ability to add broad insight into the pathobiology of AAA due to the emergence of numerous transgenic and gene knockout mice.

Creation of Murine Experimental Abdominal Aortic Aneurysms with Elastase 

We describe a surgical protocol to consistently induce robust descending thoracic aortic aneurysms in mice. The procedure involves left thoracotomy, thoracic aorta exposure, and placement of a sponge soaked in porcine pancreatic elastase on the aortic wall.

Murine Surgical Model of Topical Elastase Induced Descending Thoracic Aortic Aneurysm

This manuscript presents a protocol for establishing a mouse abdominal aortic aneurysm model using calcium chloride and elastase, combining the advantages of previous modeling methods. This model can be utilized to investigate the pathophysiological mechanisms underlying abdominal aortic aneurysms.

A Mouse Abdominal Aortic Aneurysm Model by Periadventitial Calcium Chloride and Elastase Infiltration

This protocol describes a methodical surgical approach to modeling advanced abdominal aortic aneurysms in mice by a combination of directly applying elastase to the infrarenal aorta and administering ß-aminopropionitrile through drinking water.

Advanced Abdominal Aortic Aneurysm Modeling in Mice by Combination of Topical Elastase and Oral &#223;-aminopropionitrile

The present protocol describes a standardized surgical method for the induction of carotid aneurysms in a murine model through a single surgical operation.

A Murine Model of Carotid Aneurysm Formation

This protocol provides a repeatable and reliable method for inducing abdominal aortic aneurysms (AAAs) in C57BL/6 mice using elastin sensitization and perivascular elastase application, as an AAA model that incorporates an immune-sensitization component.

Reliable Creation of a Murine Abdominal Aortic Aneurysm Model Using Elastin Sensitization and Perivascular Elastase

Orthotopic animal models of ovarian cancer replicate better human disease and therefore enhance our understanding of cancer progression and tumor response to therapy.  A mouse model receives an intrabursal injection of luciferase-expressing ovarian tumor cells.  Treatment is administered via oral gavage.  Tumor growth is monitored by in vivo imaging system.

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

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The nature of the interactions between hematopoietic stem and progenitor cells (HSPCs) and bone marrow niches is poorly understood. Custom hardware modifications and a multi-step acquisition protocol allow the use of two-photon and confocal microscopy to image ex vivo labeled HSPCs homed within bone marrow areas, tracking interactions and movement.

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

The vascular endothelial cells play a significant role in many important cardiovascular disorders. This article describes a simple method to isolate and expand endothelial cells from the mouse aorta without using any special equipment. Our protocol provides an effective means of identifying mechanisms in endothelial cell physiopathology.

Isolation and Primary Culture of Mouse Aortic Endothelial Cells

Learn a standardized surgical protocol for inducing abdominal aortic aneurysm in mice using perivascular elastase application. This method achieves high reproducibility with 91.7% incidence in females and low operational costs.

Mouse Abdominal Aortic Aneurysm Model Induced by Perivascular Application of Elastase

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