Perivascular Elastase Application
Perivascular Elastase Application - Related Videos
04:49
Mouse Abdominal Aortic Aneurysm Model Induced by Perivascular Application of Elastase
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Cited by 17 •
2022
The present protocol describes a standardized surgical method for the elastase-induced AAA model through the direct application of elastase to the adventitia of infrarenal abdominal aorta in mice.
08:54
Reliable Creation of a Murine Abdominal Aortic Aneurysm Model Using Elastin Sensitization and Perivascular Elastase
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2026
This protocol provides a repeatable and reliable method for inducing abdominal aortic aneurysms (AAAs) in C57BL/6 mice using elastin sensitization and perivascular elastase application, as an AAA model that incorporates an immune-sensitization component.
07:05
Use of Human Perivascular Stem Cells for Bone Regeneration
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Cited by 42 •
2012
Human perivascular stem cells (PSCs) are a novel stem cell class for skeletal tissue regeneration similar to mesenchymal stem cells (MSCs). PSCs can be isolated by FACS (fluorescence activated cell sorting) from adipose tissue procured during standard liposuction procedures, then combined with an osteoinductive scaffold to achieve bone formation in vivo.
10:23
Creation of Murine Experimental Abdominal Aortic Aneurysms with Elastase
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Cited by 55 •
2009
This video shows how to induce abdominal aortic aneurysms (AAA) in mice via transient intraluminal infusion of porcine pancreatic elastase into the infrarenal segment of the abdominal aorta. The model has the ability to add broad insight into the pathobiology of AAA due to the emergence of numerous transgenic and gene knockout mice.
09:05
A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells
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Cited by 4 •
2017
Here we present a novel clinical grade isolation and culture method for kidney Perivascular Stromal Cells (kPSCs) based on whole organ perfusion with digestive enzymes and NG2-cell enrichment. With this method, it is possible to acquire sufficient cell numbers for cellular therapy.
FAQs
Elastin normally contributes to the vessel wall’s structural support. When elastase degrades this component, the wall becomes more vulnerable to weakening and dilation. This loss also creates a setting in which inflammation and extracellular matrix remodeling can influence disease progression. Studying these linked changes helps investigators connect matrix damage with the development of vascular wall instability.
Elastase-induced structural injury is accompanied by inflammatory activity and remodeling of the extracellular matrix, the network of molecules supporting vascular tissue. These responses can alter interactions among vascular cells and matrix components rather than acting as isolated events. Examining them together allows researchers to investigate how tissue responses reinforce weakening and progressive enlargement of the vessel.
Applying elastase around a selected blood vessel creates a localized experimental setting for examining vascular remodeling. This focus helps investigators relate regional matrix injury to subsequent changes in vessel structure, inflammation, and dilation. The controlled location also supports comparisons of biological responses, disease pathways, or interventions within a defined vascular context rather than examining nonspecific changes throughout the organism.
A study generally establishes the vascular site, applies elastase around the vessel, and then follows the resulting structural and biological changes. Investigators can evaluate vessel dilation alongside inflammation and extracellular matrix remodeling as the model progresses. The workflow therefore links a defined experimental exposure with measurable features of vascular instability and provides a basis for comparing experimental groups.
Key findings include changes in vessel diameter, loss or remodeling of extracellular matrix support, inflammatory responses, and evidence of increasing wall instability. Researchers may also use imaging methods to follow structural changes over time. Together, these outcomes help characterize aneurysm development and determine whether a genetic factor, disease pathway, or treatment alters the remodeling process.
The model provides a controlled platform for investigating how vascular cells, matrix components, and disease-related pathways interact during vessel weakening. It can support studies of genetic factors, evaluation of imaging methods, and testing of potential therapies for vascular disorders. By connecting molecular or cellular mechanisms with vessel-level changes, the approach helps assess both disease biology and experimental interventions.