Biology
A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.
The JoVE video player is compatible with HTML5 and Adobe Flash. Older browsers that do not support HTML5 and the H.264 video codec will still use a Flash-based video player. We recommend downloading the newest version of Flash here, but we support all versions 10 and above.
If that doesn't help, please let us know.
Mouse Abdominal Aortic Aneurysm Model Induced by Perivascular Application of Elastase
Chapters
Summary February 11th, 2022
The present protocol describes a standardized surgical method for the elastase-induced AAA model through the direct application of elastase to the adventitia of infrarenal abdominal aorta in mice.
Transcript
This is a standardized protocol for elastase induced abdominal aortic aneurysm, which would greatly enhance the reproducibility and the consistency of studies using this model. This technique has a consistent induction rate of abdominal aortic aneurysm. It also owns the advantage of low expenses and short operation time, which will result in stronger statistical power and the reproducibility.
You need to ensure the connective tissues covering the aorta were removed so that the elastase will have a better chance of penetration, but be careful not to damage the vessels. To begin, administer five milligrams per kilograms of carprofen subcutaneously, 30 minutes before the induction of anesthesia. Test the anesthetized mouse for the lack of toe pinch response before skin incision.
Make a 2.5 centimeter longitudinal incision on the skin, along the midline of the middle and lower abdomen. Gently pull up the underlying muscle and make a 2.5 centimeter longitudinal incision along linea alba. Use wetted cotton tipped applicators to move the intestines and stomach to the right side of the mouse, and access the abdominal cavity.
Use forceps to gently remove the connective tissue covering the abdominal aorta and inferior vena cava. Use forceps to gently dissect the backside of the abdominal aorta and inferior vena cava from the underlying muscles. Place a piece of four centimeters by four millimeters glove stripe through the backside of the abdominal aorta and inferior vena cava, and straighten the stripe.
Place the stripe approximately 0.5 centimeters away from the right renal artery. Above the stripe, place a piece of three centimeters by two millimeters cotton pad through the backside of the abdominal aorta and inferior vena cava. Then, straighten the cotton pad.
To incubate the elastase, use a pipette to drop 30 microliters of porcine pancreatic elastase onto the aorta segment above the cotton pad. Wrap the cotton pad and stripe around the aorta and inferior vena cava. Rinse a piece of 10 centimeters by 10 centimeters gauze with sterile 0.9%saline and place it on the abdomen.
After 30 minutes, remove the stripe and cotton pad with forceps. Irrigate the aorta and abdominal cavity with 500 microliters of sterile 0.9%saline. Use gauze to absorb the remaining saline.
Re-approximate the muscle layers with a running 6-0 non-absorbable monofilament suture. Then, close the skin with three to four interrupted at 6-0 non-absorbable monofilament sutures. The incidence rate of developing an abdominal aortic aneurysm on postoperative day 14 was 91.7%for female animals, while the incidence rate for male animals was 72.7%Among the 19 animals in which abdominal aortic aneurysm was developed, a 1.7 fold and 1.6 fold increase of maximum abdominal aortic diameter was observed, in females and males respectively, compared to non-surgery controls.
Representative images of the aorta from non-surgery and surgery groups on a post-operative day 14 are displayed. This technique served as a potential tool to help dissect the pathophysiology of triple A and help for the discovery of new potential therapeutic targets.
Related Videos
You might already have access to this content!
Please enter your Institution or Company email below to check.
has access to
Please create a free JoVE account to get access
Login to access JoVE
Please login to your JoVE account to get access
We use/store this info to ensure you have proper access and that your account is secure. We may use this info to send you notifications about your account, your institutional access, and/or other related products. To learn more about our GDPR policies click here.
If you want more info regarding data storage, please contact gdpr@jove.com.
Please enter your email address so we may send you a link to reset your password.
We use/store this info to ensure you have proper access and that your account is secure. We may use this info to send you notifications about your account, your institutional access, and/or other related products. To learn more about our GDPR policies click here.
If you want more info regarding data storage, please contact gdpr@jove.com.
Your JoVE Unlimited Free Trial
Fill the form to request your free trial.
We use/store this info to ensure you have proper access and that your account is secure. We may use this info to send you notifications about your account, your institutional access, and/or other related products. To learn more about our GDPR policies click here.
If you want more info regarding data storage, please contact gdpr@jove.com.
Thank You!
A JoVE representative will be in touch with you shortly.
Thank You!
You have already requested a trial and a JoVE representative will be in touch with you shortly. If you need immediate assistance, please email us at subscriptions@jove.com.
Thank You!
Please enjoy a free 2-hour trial. In order to begin, please login.
Thank You!
You have unlocked a 2-hour free trial now. All JoVE videos and articles can be accessed for free.
To get started, a verification email has been sent to email@institution.com. Please follow the link in the email to activate your free trial account. If you do not see the message in your inbox, please check your "Spam" folder.
