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Journal / Biology / 基于CRISPR/Cas9技术构建迁徙蝗虫纯合突变体…
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基于CRISPR/Cas9技术构建迁徙蝗虫纯合突变体
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基于CRISPR/Cas9技术构建迁徙蝗虫纯合突变体

Article DOI: 10.3791/63629-v • 10:07 min • March 16th, 2022
March 16th, 2022 •
*1, *2,3, 2,3, 1, 2,3, 2,3, 2, 2
1State Key Laboratory of Cotton Biology, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 2Research Institute of Applied Biology, Shanxi University, 3School of Life Science, Shanxi University
* These authors contributed equally

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本研究为基于CRISPR/Cas9核糖核酸酶构建纯合蝗虫突变体提供了系统优化的程序，以及蝗虫卵冷冻保存和复苏的详细方法。

Tags

撤稿，第181期，纯合突变体，迁徙蝗虫，CRISPR / Cas9，基因组编辑，显微注射，卵子冷冻保存和复苏
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