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Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus

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Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus

Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus

DOI:

10.3791/63645-v

•

06:54 min

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March 09, 2022

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Xiaofang Liu, Xiang Zhou, Lifeng Zhou, Jiafu Hu, Kai Guo

¹College of Forestry and Biotechnology,Zhejiang Agricultural & Forestry University

Chapters

00:04Introduction
00:39Gene Cloning
03:13RNAi by Soaking Method
04:40Body Length Evaluation of Nematode Adults after RNAi
05:21Results: Effect of ppm-1 RNAi on B. xylophilus
06:07Conclusion

Summary

Automatic Translation

Here, we introduce a detailed soaking method of RNA interference in Bursaphelenchus xylophilus to facilitate the study of gene functions.

Tags

Keywords: RNA Interference RNAi Bursaphelenchus Xylophilus Pinewood Nematode Gene Function Nematode Control Baermann Funnel DsRNA In Vitro Transcription Soaking Ppm-1 Gene

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