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Genome-wide Profiling of Transcription Factor-DNA Binding Interactions in Candida albicans: A Comprehensive CUT&RUN Method and Data Analysis Workflow
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Genome-wide Profiling of Transcription Factor-DNA Binding Interactions in Candida albicans: A Comprehensive CUT&RUN Method and Data Analysis Workflow

Genome-wide Profiling of Transcription Factor-DNA Binding Interactions in Candida albicans: A Comprehensive CUT&RUN Method and Data Analysis Workflow

DOI:
10.3791/63655-v

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07:48 min

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April 01, 2022

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, , , , , ,
1Department of Molecular and Cell Biology,University of California, Merced, 2Quantitative and Systems Biology Graduate Program,University of California, Merced, 3Department of Applied Mathematics,University of California, Merced, 4Health Sciences Research Institute,University of California, Merced

Chapters

  • 00:04Introduction
  • 00:49Isolation of Nuclei and Primary Antibody Binding
  • 02:40Binding of pAG-MNase to Antibody
  • 03:36Library Preparation for Sequencing
  • 05:22CUT&RUN Sequence Analysis
  • 05:54Results: Quality Control Steps and Evaluation of Ndt80 and Efg1 Enriched Peaks Using CUT&RUN Protocol
  • 07:22Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes an experimental method and data analysis workflow for cleavage under targets and release using nuclease (CUT&RUN) in the human fungal pathogen Candida albicans.

Tags

Genome-wide ProfilingTranscription Factor-DNA BindingCandida AlbicansCUT&RUNChIP-chipChIP-seqEpitope TaggingNuclei IsolationFluorescence MicroscopyAntibody IncubationProtein AG-MNaseData Analysis Workflow

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