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Genome-wide Profiling of Transcription Factor-DNA Binding Interactions in Candida albicans: A Comprehensive CUT&RUN Method and Data Analysis Workflow
JoVE Journal
Biology
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JoVE Journal Biology
Genome-wide Profiling of Transcription Factor-DNA Binding Interactions in Candida albicans: A Comprehensive CUT&RUN Method and Data Analysis Workflow
DOI:

07:48 min

April 01, 2022

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Chapters

  • 00:04Introduction
  • 00:49Isolation of Nuclei and Primary Antibody Binding
  • 02:40Binding of pAG-MNase to Antibody
  • 03:36Library Preparation for Sequencing
  • 05:22CUT&RUN Sequence Analysis
  • 05:54Results: Quality Control Steps and Evaluation of Ndt80 and Efg1 Enriched Peaks Using CUT&RUN Protocol
  • 07:22Conclusion

Summary

Automatic Translation

This protocol describes an experimental method and data analysis workflow for cleavage under targets and release using nuclease (CUT&RUN) in the human fungal pathogen Candida albicans.

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