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Full- versus Sub-Regional Quantification of Amyloid-Beta Load on Mouse Brain Sections
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Full- versus Sub-Regional Quantification of Amyloid-Beta Load on Mouse Brain Sections

Full- versus Sub-Regional Quantification of Amyloid-Beta Load on Mouse Brain Sections

DOI:
10.3791/63669-v

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07:28 min

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May 19, 2022

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, , , ,
1Henry E. Riggs School of Applied Life Sciences,Keck Graduate Institute, 2Crean College of Health and Behavioral Sciences,Chapman University, 3Keck Science Department,Claremont McKenna College, 4Department of Biomedical and Pharmaceutical Sciences, School of Pharmacy,Chapman University, 5Department of Neurology,University of California, Irvine

Chapters

  • 00:05Introduction
  • 00:42Full-Region of Interest (ROI) Analysis
  • 03:28Sub-Region of Interest (ROI) Analysis
  • 05:19Results: Impact of Size of ROI on Aβ-Positive Area Quantification in the APP/PS1 Transgenic Mouse of Alzheimer’s Disease
  • 06:50Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes and compares the procedure to perform a full-region or sub-region of interest analysis of sagittal mouse brain sections to quantify amyloid-beta load in the APP/PS1 transgenic mouse model of Alzheimer's disease.

Tags

Keywords: Amyloid-betaPlaque LoadAPP/PS1 Transgenic MiceImage AnalysisFull Region Of InterestSub-region Of InterestScale BarBrightnessContrastHippocampusCortex6-E10 StainingColor Thresholding

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