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Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway Organoids
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Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway Organoids

Article DOI: 10.3791/63684-v • 10:12 min • March 23rd, 2022
March 23rd, 2022 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2,3
1Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 2Centre for Virology, Vaccinology and Therapeutics, Hong Kong Science and Technology Park, 3State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong

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Summary
Automatic Translation

The protocol presents a method to derive human lung organoids from primary lung tissues, expand the lung organoids and induce proximal differentiation to generate 3D and 2D airway organoids that faithfully phenocopy the human airway epithelium.

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Human Lung Organoids Proximal Differentiation Mature Airway Organoids Culture Plates Primary Lung Tissues High Efficiency Stably Expanded Near Physiological Level Cell Isolation 3D Organoid Culture Mince Lung Tissues Sterile Scalpel Cold Basal Medium Centrifuge Tissues Collagenase Final Concentration Digestion Serological Pipette
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