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Journal / Neuroscience / Ter plaatse Hybridisatie gecombineerd met…
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JoVE Journal Neuroscience
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Ter plaatse Hybridisatie gecombineerd met immunohistochemie in cryosectie zebravisembryo's

Article DOI: 10.3791/63715-v • 07:36 min • March 3rd, 2022
March 3rd, 2022 •
*1,2, *3, 2, 4, 2, 1, 2,3, 5, 1
1School of Life Sciences, Nantong University, 2Medical School, Nantong University, 3Key Laboratory of Neuroregeneration of Jiangsu Province and Ministry of Education, Co-Innovation Center of Neuroregeneration, Nantong University, 4Nantong Maternal and Child Health Care Hospital, 5Laboratory Animals Center, Nantong University
* These authors contributed equally

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Dit protocol beschrijft hoe beelden kunnen worden verkregen door in situ hybridisatie en immunohistochemie van embryonale secties van zebravissen te combineren. In situ hybridisatie werd uitgevoerd voorafgaand aan cryosectie, gevolgd door antilichaamkleuring. Het is nuttig om de expressiepatronen van twee genen in zebravissen te detecteren als er een tekort aan antilichamen is.

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Deze maand in JoVE Nummer 181 In situ hybridisatie immunohistochemie zebravis mRNA antilichaam eiwit
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