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Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents

Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents

DOI:
10.3791/63723-v

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08:39 min

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May 16, 2022

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, , ,
1Danish Headache Center, Department of Neurology,Rigshospitalet, University of Copenhagen, 2EHESP, Irset (Institut de Recherche en Santé, Environnement et Travail),Univ Rennes, Inserm, 3Department of Biology, Section of Cell Biology and Physiology,University of Copenhagen

Chapters

  • 00:04Introduction
  • 00:43Preparing Rat Tissue
  • 02:05Preparing Mouse Tissue
  • 03:16Washing and Preparation of Rat and Mouse Tissue
  • 04:21Preparation of Sample Collecting Tubes and Test Solutions
  • 04:46Determining Basal CGRP Release Levels
  • 05:26Performing Concentration‐Response Stimulation, Positive Control for the Experiment, and Measurement of CGRP Contractions
  • 06:29Results: Capsaicin‐Induced and TRPA1‐Dependent CGRP Release from Rat and Mouse Tissue
  • 08:04Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes the ex vivo calcitonin gene-related peptide (CGRP) release model and the strategy to quantify the effect of pharmacological agents on the amount of CGRP released from the trigeminovascular system in rodents.

Tags

Keywords: Ex VivoCalcitonin Gene-related PeptideTrigeminovascular SystemRodentsTrigeminal Vascular SystemCGRP ReleaseTrigeminal NerveTrigeminal GanglionTrigeminal Nucleus CaudalisBrain DissectionBrainstemCerebellumCranium

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