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JoVE Journal Developmental Biology
Generation of Mesenchymal Stem Cells from Human Umbilical Cord Tissue and their Differentiation into the Skeletal Muscle Lineage
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Generation of Mesenchymal Stem Cells from Human Umbilical Cord Tissue and their Differentiation into the Skeletal Muscle Lineage

Article DOI: 10.3791/63725-v • 07:27 min • August 31st, 2022
August 31st, 2022 •
1, 1
1Translational Health Science and Technology Institute (THSTI), NCR Biotech Science Cluster

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Summary
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We describe a protocol for the isolation of mesenchymal stem cells from human umbilical cord tissue and their differentiation into the skeletal muscle lineage.

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Mesenchymal Stem Cells Human Umbilical Cord Tissue Skeletal Muscle Lineage Isolation Protocol Differentiation Method Viability Contamination Myogenic Differentiation Wharton's Jelly Endothelial Cells Cord Torsion Mucoid Consistency Tissue Culture Dish PBS Rinse Umbilical Arteries Umbilical Veins Blood Vessels Removal Tissue Fragmentation
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