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Ensemble Force Spectroscopy by Shear Forces
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Bioengineering
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JoVE Journal Bioengineering
Ensemble Force Spectroscopy by Shear Forces

Ensemble Force Spectroscopy by Shear Forces

DOI:
10.3791/63741-v

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07:30 min

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July 26, 2022

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, ,
1Department of Chemistry & Biochemistry,Kent State University

Chapters

  • 00:04Introduction
  • 00:46Preparation of the Shear Force Microscope
  • 02:11Unfolding I-Motifs with and without Ligands
  • 03:47Shear Force-Actuated Click Reaction
  • 05:29Results: Real-Time Investigation of the Unfolding of an Ensemble Set of Biomolecular Structures by Shear Force
  • 06:39Conclusion

Summary

Automatic Translation

Automatic Translation

Ensemble force spectroscopy (EFS) is a robust technique for mechanical unfolding and real-time sensing of an ensemble set of biomolecular structures in biophysical and biosensing fields.

Tags

Keywords: Ensemble Force SpectroscopyShear ForcesHigh ThroughputBiomolecular StructuresMechanical Chemical PropertiesMolecular EnsemblesFluorescence MicroscopeHomogenizerReaction ChamberI-motif DNALigand L2H24OTD’Background Fluorescence

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