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Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers

Article DOI: 10.3791/63758-v • 09:36 min • July 28th, 2022
July 28th, 2022 •
*1, *1, 1, 1
1Department of Pediatrics, Division of Neonatal-Perinatal Medicine, University of Oklahoma Health Sciences Center
* These authors contributed equally

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Summary
Automatic Translation

This protocol describes a method to establish and perform a scratch wound assay on two-dimensional (2D) monolayers derived from three-dimensional (3D) enteroids isolated from non-human primate ileum.

Tags

Hyaluronic Acid 35 KDa In Vitro Model Preterm Small Intestinal Injury Enteroid-derived Monolayers Scratch Wound Assay Enteroid Monolayer Model Intestinal Regeneration Extracellular Matrix ECM-based Hydrogel Tissue Culture Plate Cell Dissociation Reagent
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