Journal
/
Medicine
/
Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers

Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers

DOI:
10.3791/63758-v

•

09:36 min

•

July 28, 2022

•

, , ,
1Department of Pediatrics, Division of Neonatal-Perinatal Medicine,University of Oklahoma Health Sciences Center

Chapters

  • 00:04Introduction
  • 00:46Enteroid Monolayer Formation
  • 03:47Monolayer Treatment and Scratch Wound Assay
  • 06:36Scratch Wound Analysis
  • 07:38Results: Enteroid Generation and Enteroid-Derived Monolayer Wound Healing Assay Procedure, Images, Analysis, and the Effect of HA35 on Wound Healing
  • 08:58Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a method to establish and perform a scratch wound assay on two-dimensional (2D) monolayers derived from three-dimensional (3D) enteroids isolated from non-human primate ileum.

Tags

Hyaluronic AcidPretermSmall IntestineEnteroidMonolayerWound HealingRegenerationIn Vitro ModelCell CultureExtracellular MatrixCell DissociationTrypsinDMEM/F-122D Model

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

A Contusive Model of Unilateral Cervical Spinal Cord Injury Using the Infinite Horizon Impactor

Murine Model of Wound Healing

An Alkali-burn Injury Model of Corneal Neovascularization in the Mouse

Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation

Development of an Algorithm to Perform a Comprehensive Study of Autonomic Dysreflexia in Animals with High Spinal Cord Injury Using a Telemetry Device

Murine Model of Intestinal Ischemia-reperfusion Injury

Open Tracheostomy Gastric Acid Aspiration Murine Model of Acute Lung Injury Results in Maximal Acute Nonlethal Lung Injury

Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells

In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur

Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia

Read Article