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Journal / Biochemistry / 一种通过牛磺胆酸钠共转运多肽作为治疗靶点检查乙型肝炎病毒进入的感受态肝细胞模型…
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Biochemistry
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JoVE Journal Biochemistry
一种通过牛磺胆酸钠共转运多肽作为治疗靶点检查乙型肝炎病毒进入的感受态肝细胞模型
Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
Journal (Biochemistry)
Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
Journal (Biochemistry)
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
 
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一种通过牛磺胆酸钠共转运多肽作为治疗靶点检查乙型肝炎病毒进入的感受态肝细胞模型

Article DOI: 10.3791/63761-v • 11:34 min • May 10th, 2022
May 10th, 2022 •
1, 2, 2, 3,4, 5
1Department of Biochemistry, Faculty of Pharmacy, Mahidol University, 2Program in Translational Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, 3Excellent Center for Drug Discovery, Faculty of Science, Mahidol University, 4Department of Biotechnology, Faculty of Science, Mahidol University, 5Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University

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我们提出了一种针对病毒进入前和进入生命周期阶段的抗乙型肝炎病毒（HBV）化合物的协议，使用等温滴定量热法测量与宿主牛磺胆酸钠共转运多肽的结合亲和力（KD）。通过抑制病毒生命周期标志物（cccDNA形成，转录和病毒组装）来确定抗病毒功效。

Tags

生物化学，第183期，乙型肝炎，进入抑制剂，肝细胞，NTCP，HBV，ITC，结合测定，TCA摄取，等温滴定量热法
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