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En kompetent hepatocyttmodell som undersøker hepatitt B-virusinngang gjennom natriumtaurokolat som transporterer polypeptid som terapeutisk mål
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1Department of Biochemistry, Faculty of Pharmacy,Mahidol University, 2Program in Translational Medicine, Faculty of Medicine Ramathibodi Hospital,Mahidol University, 3Excellent Center for Drug Discovery, Faculty of Science,Mahidol University, 4Department of Biotechnology, Faculty of Science,Mahidol University, 5Department of Pharmacology, Faculty of Medicine Siriraj Hospital,Mahidol University
Chapters
- 00:05Introduction
- 00:50Measuring the Sodium Taurocholate Co-Transporting Polypeptide (NTCP) Using Flow Cytometry
- 04:30Anti-HBV (Hepatitis B Virus) Entry Assay
- 06:13HBV (Hepatitis B Virus) -Cell Binding Assay
- 06:50Taurocholic Acid Uptake Assay
- 07:34Determination of Protein-Ligand Interactions Using Isothermal Titration Calorimetry
- 09:14Results: Screening of Anti-Hepatitis B Virus Using Isothermal Titration Calorimetry
- 10:36Conclusion
Vi presenterer en protokoll for å screene anti-hepatitt B-virus (HBV) forbindelser rettet mot pre- og post-viral oppføring livssyklus stadier, ved hjelp av isotermisk titrering kalorimetri for å måle binding affinitet (KD) med vert natrium taurocholate cotransporting polypeptid. Antiviral effekt ble bestemt gjennom undertrykkelse av virale livssyklusmarkører (cccDNA-dannelse, transkripsjon og viral montering).
