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Journal / Medicine / 用于检测人类精子功能缺陷的生物标志物的流式细胞术分析…
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用于检测人类精子功能缺陷的生物标志物的流式细胞术分析
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用于检测人类精子功能缺陷的生物标志物的流式细胞术分析

Article DOI: 10.3791/63790-v • 08:48 min • April 21st, 2022
April 21st, 2022 •
1, 1, 1, 1, 2, 1, 1, 1, 1, 1
1Institute of Toxicology, College of Preventive Medicine, Army Medical University (Third Military Medical University), 2Department of Chemical Defense Medicine, College of Military Preventive Medicine, Army Medical University (Third Military Medical University)

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本方案提供了一种实用的解决方案，允许使用单个细胞仪测量人类精子中的细胞凋亡、线粒体膜电位和DNA损伤。

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流式细胞术、生物标志物、精子功能缺陷、常规精液参数、特发性不孕症、精子凋亡、线粒体膜电位 （MMP）、DNA 损伤、FCM 技术、膜联蛋白 V-荧光素异硫氰酸酯 （FITC）/碘化丙啶 （PI） 染色、5,5& 8242;、6,6& 8242;-四氯-1,1& 8242;、3,3& 8242;-四乙基苯并咪唑基羰花青碘化物 （JC-1） 探针、精子染色质结构测定 （SCSA）、吖啶橙 （AO） 染色
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