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Journal / Medicine / İnsan Sperm Fonksiyonel Kusurlarını Tespit Etmek…
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JoVE Journal Medicine
İnsan Sperm Fonksiyonel Kusurlarını Tespit Etmek için Biyobelirteçlerin Akış Sitometrik Analizi
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İnsan Sperm Fonksiyonel Kusurlarını Tespit Etmek için Biyobelirteçlerin Akış Sitometrik Analizi

Article DOI: 10.3791/63790-v • 08:48 min • April 21st, 2022
April 21st, 2022 •
1, 1, 1, 1, 2, 1, 1, 1, 1, 1
1Institute of Toxicology, College of Preventive Medicine, Army Medical University (Third Military Medical University), 2Department of Chemical Defense Medicine, College of Military Preventive Medicine, Army Medical University (Third Military Medical University)

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Mevcut protokol, insan sperminde apoptozis, mitokondriyal membran potansiyeli ve DNA hasarının tek bir sitometre ile ölçülmesine olanak sağlayan pratik bir çözüm sunmaktadır.

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Flow Sitometri Biyobelirteçler Sperm Fonksiyonel Defektleri Konvansiyonel Semen Parametreleri İdiyopatik İnfertilite Sperm Apoptozu Mitokondriyal Membran Potansiyeli (MMP) DNA Hasarı FCM Teknikleri Annexin V-floresein İzotiyosiyanat (FITC)/propidyum İyodür (PI) Boyama 5,5& 8242;,6,6&8242;-tetrakloro-1,1&8242;,3,3&8242;-tetraetil-benzimidolilkarbosiyanin İyodür (JC-1) Probu Sperm Kromatin Yapı Testi (SCSA) Akridin Turuncu (AO) Boyama
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