Journal
/
Neuroscience
/
厚自由漂浮组织切片中星形胶质细胞区域体积和瓷砖的分析
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Analysis of Astrocyte Territory Volume and Tiling in Thick Free-Floating Tissue Sections
Please note that all translations are automatically generated. Click here for the English version.

厚自由漂浮组织切片中星形胶质细胞区域体积和瓷砖的分析

DOI:
10.3791/63804-v

•

10:53 min

•

April 20, 2022

•

,
1Neuroscience Center,University of North Carolina, Chapel Hill, 2Department of Cell Biology and Physiology,University of North Carolina, Chapel Hill

Chapters

  • 00:05Introduction
  • 00:55Immunostaining
  • 04:42Confocal Imaging
  • 05:57Image Analysis
  • 09:22Results: Analysis of Astrocyte Territory Volume and Astrocyte Territory Overlap or Tiling
  • 10:27Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了用于切片，染色和成像小鼠大脑的自由浮动组织切片的方法，随后详细描述了星形胶质细胞区域体积和星形胶质细胞区域重叠或平铺的分析。

Tags

AstrocyteAstrocyte TerritoryAstrocyte MorphologyImmunohistochemistryAstrocyte DevelopmentAstrocyte-astrocyte InteractionTriton XBlocking SolutionPrimary AntibodySecondary Antibody

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

双荧光原位杂交食脑切片

一个快速高分辨率的荧光成像的方法在半厚的脑片

视网膜节的形态分析

解剖，文化与分析<em>非洲爪蟾</em>胚胎视网膜组织

免疫组化分析大鼠中枢神经系统和周围淋巴结组织切片

在缺血样条件下监测星形胶质细胞的反应性和增殖体外

厚脑切片使用高尔基考克斯方法中成像神经元

使用生物素化测定原代星形细胞培养物中蛋白质的细胞表面表达和吞噬率

一种新的体外活体成像法测定星形胶质细胞的吞噬作用 pH 指标-共轭突

通过自由浮动免疫荧光测定,在皮外神经纤维中定位阿尔法核糖核酸聚合体

Read Article