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Developmental Biology

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JoVE Journal Developmental Biology
Gerando esferas 3D e culturas de interface ar-líquido 2D de células-tronco pluripotentes induzidas pelo homem
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Gerando esferas 3D e culturas de interface ar-líquido 2D de células-tronco pluripotentes induzidas pelo homem

Article DOI: 10.3791/63875-v • 07:02 min • April 15th, 2022
April 15th, 2022 •
*1,2,3, *1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, *1,2, *1,2
1Center for Regenerative Medicine, Boston University and Boston Medical Center, 2The Pulmonary Center and Department of Medicine, Boston University School of Medicine, 3QIMR Berghofer Medical Research Institute
* These authors contributed equally

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O presente protocolo descreve células pluripotentes pluripotentes induzidas por células-tronco derivadas do tipo 2 (iAT2s). Essas células podem ser cultivadas como esferas de auto-renovação na cultura 3D ou adaptadas à cultura de interface ar-líquido (ALI).

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Biologia do Desenvolvimento Edição 182
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