Name: A Modified Vessel-Sparing Microsurgical Vasoepididymostomy (Video) | JoVE Journal
Uploaded: 2022-06-08T14:20:00.000+00:00
Duration: 4 min 59 s
Description: Learn a modified vessel-sparing microsurgical vasoepididymostomy technique for urology and reproductive surgery. This protocol preserves vasal vessels to enhance safety, maintain physiological structures, and ensure high patency rates during fertility restoration procedures.

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5 minutes

I would like to thank Professor Long Tian (Department of Urology, Beijing Chaoyang Hospital) for the technical instruction on vasoepididymostomy. This modified procedure I designed is inspired by his artery-sparing microsurgical vasoepididymostomy. I would also like to thank Dr. Moqi Lv (Medical School, Xi&#39;an Jiaotong University) for the help with polishing the present paper.

This study was approved by the Ethics Committee of Northwest Women&#39;s and Children&#39;s Hospital (No. 2021002).
1. Preparations
<ol>
	<li>Place the patient supine on the operating table and perform anesthesia as per the anesthetist&#39;s recommendations.</li>
	<li>Insert a 16Fr Foley catheter around the incision site after the field is disinfected and covered with surgical drapes.</li>
</ol>
2. Modified vessel-sparing LIVE
<ol>
	<li>Create a 3 cm vertical incision in the middle of the scrotum and deliver the testis from the incision.</li>
	<li>Open the tunica vaginalis. Carefully dissociate the vas deferens and adjacent vessels. The dissociated length is ~5 cm (Figure 1).</li>
	<li>Place an operating microscope and ready the micro instruments: a micro needle holder, micro scissors, and micro forceps. Execute the following steps using the operating microscope.</li>
	<li>Create a buttonhole of a diameter of 5 mm at the tunica of cauda epididymis using micro scissors. Identify a dilated epididymal tubule and carefully dissociate it using micro forceps. After that, keep it for anastomosis.</li>
	<li>Place two needles of double-armed 11-0 microsutures longitudinally in the selected epididymal tubule, and open the epididymal tubule longitudinally between the two needles using a 15&#176; ophthalmic knife. Ensure that the opening length does not exceed the length between the entry point and the exit point of the suture on the epididymal tubule (Figure 2).</li>
	<li>Sample some epididymal fluid flowing from the incision in the tubule with a glass slide and hand it to an examiner to check for sperm. Gently pull out two needles in the epididymal tubule separately if sperm are found.</li>
	<li>Make a puncture in the gap between the vas deferens and adjacent vessels using deferens separating forceps. The puncturing site is approximately 1 cm away from the junction of the cauda epididymis and the vas deferens. Completely transect the vas deferens above the deferens separating forceps with a knife, and preserve the vasal vessels below the deferens separating forceps (Figure 3).</li>
	<li>Cannulate the 24-G angiocatheter sheath into the vas deferens lumen on the distal testicular side, followed by a bolus injection of diluted methylene blue. Observe the color of the urine. Blue staining of the urine indicates distal patency.</li>
	<li>Dissociate the vas deferens on the proximal testicular side using micro scissors to separate it from the vasal vessels. Ensure that the dissociation is executed along the vas deferens side to avoid damage to the vasal vessels. The dissociated length is ~2-3 cm. Then, remove the dissociated vas deferens and ligate the broken end of the vas deferens. During the dissociation, stop the bleeding using bipolar electrocoagulation or by ligating some small branch vessels using a 5-0 silk thread. This operation is called retrograde dissociation (Figure 4 and Figure 5).</li>
	<li>Carefully separate the vas deferens on the distal testicular side from the vasal vessels using deferens separating forceps combined with micro scissors. The dissociated length is ~0.5 cm. Separate the vas deferens and the vasal vessels without damaging the vas deferens or the vasal vessels. This operation is called anterograde dissociation (Figure 4 and Figure 6).</li>
	<li>Fix the vas deferens to be anastomosed with the tunica vaginalis using 8-0 microsutures at the included angle between the vas deferens and adjacent vessels. After fixation, ensure that the lumen of the vas deferens reaches the anastomosis site with no significant tension. This is regarded as the first-stage tension reduction.</li>
	<li>Fix the muscularis edge of the vas deferens and epididymis tunica using 3-4 interrupted 8-0 microsutures. This is regarded as the second-stage tension reduction.</li>
	<li>Pass the four needles of 11-0 microsutures in the epididymal tubule through four points (Figure 7) on the cutting surface of the vas deferens in an inside-out fashion. Then, perform the intussusception of the epididymal tubule into the vas deferens8.</li>
	<li>Suture the muscularis edge of the vas deferens and the epididymal tunica using 10-12 interrupted sutures of 8-0 microsutures.</li>
	<li>Fix the broken end of the vas deferens on the tunica vaginalis using 3-4 interrupted sutures of 8-0 microsutures (Figure 4 and Figure 8). Ensure that the free vasal vessels are fixed and cannot move. This operation is called fixation of the vasal vessels.</li>
	<li>Check whether there is tension in the vas deferens and whether there is tension and bleeding in the preserved vasal vessels. Then, suture each layer of tissue sequentially and terminate the operation.</li>
</ol>
3. Postoperative management
<ol>
	<li>Avoid sexual intercourse for 1 month after surgery.</li>
	<li>Wear a scrotal supporter or tight underpants for 1 week after surgery, and avoid standing or walking for a long time.</li>
	<li>Take antibiotics for 1 week to prevent infection.</li>
</ol>

<ol><li>Wagenknecht, L. V., Klosterhalfen, H., Schirren, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microsurgery+in+andrologic+urology.+I.Refertilization%5BTitle%5D)+AND+%22Journal+of+Microsurgery%22%5BJournal%5D)">Microsurgery in andrologic urology. I.Refertilization.</a> Journal of Microsurgery. 1 (5), 370-376 (1980).</li>
<li>Thomas, A. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Vasoepididymostomy%5BTitle%5D)+AND+%22Urologic+Clinics+of+North+America%22%5BJournal%5D)">Vasoepididymostomy.</a> Urologic Clinics of North America. 14 (3), 527-538 (1987).</li>
<li>Berger, R. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Triangulation+end-to-side+vasoepididymostomy%5BTitle%5D)+AND+%22The+Journal+of+Urology%22%5BJournal%5D)">Triangulation end-to-side vasoepididymostomy.</a> The Journal of Urology. 159 (6), 1951-1953 (1998).</li>
<li>Marmar, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Modified+vasoepididymostomy+with+simultaneous+double+needle+placement%2C+tubulotomy+and+tubular+invagination%5BTitle%5D)+AND+%22The+Journal+of+Urology%22%5BJournal%5D)">Modified vasoepididymostomy with simultaneous double needle placement, tubulotomy and tubular invagination.</a> The Journal of Urology. 163 (2), 483-486 (2000).</li>
<li>Chan, P. T., Li, P. S., Goldstein, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microsurgical+vasoepididymostomy%3A+A+prospective+randomized+study+of+3+intussusception+techniques+in+rats%5BTitle%5D)+AND+%22The+Journal+of+Urology%22%5BJournal%5D)">Microsurgical vasoepididymostomy: A prospective randomized study of 3 intussusception techniques in rats.</a> The Journal of Urology. 169 (5), 1924-1929 (2003).</li>
<li>Chan, P. T. Vasoepididymostomy. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aChan%2C+PT+%22Glenn%27s+Urologic+Surgery%2C+7th+ed%22">Glenn's Urologic Surgery, 7th ed</a>. Graham, S. D., Keane, T. E. , Lippincott Williams & Wilkins. Philadelphia, PA. 379-386 (2009).</li>
<li>Chan, P. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+evolution+and+refinement+of+vasoepididymostomy+techniques%5BTitle%5D)+AND+%22Asian+Journal+of+Andrology%22%5BJournal%5D)">The evolution and refinement of vasoepididymostomy techniques.</a> Asian Journal of Andrology. 15 (1), 49-55 (2013).</li>
<li>Mostafa, T., Labib, I., El-Khayat, Y., El-Rahman El-Shahat, A., Gadallah, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Human+testicular+arterial+supply%3A+Gross+anatomy%2C+corrosion+cast%2C+and+radiologic+study%5BTitle%5D)+AND+%22Fertility+and+Sterility%22%5BJournal%5D)">Human testicular arterial supply: Gross anatomy, corrosion cast, and radiologic study.</a> Fertility and Sterility. 90 (6), 2226-2230 (2008).</li>
<li>Zhang, Y., Wu, X., Yang, X. J., Zhang, H., Zhang, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Vasal+vessels+preserving+microsurgical+vasoepididymostomy+in+cases+of+previous+varicocelectomy%3A+A+case+report+and+literature+review%5BTitle%5D)+AND+%22Asian+Journal+of+Andrology%22%5BJournal%5D)">Vasal vessels preserving microsurgical vasoepididymostomy in cases of previous varicocelectomy: A case report and literature review.</a> Asian Journal of Andrology. 18 (1), 154-156 (2016).</li>
<li>Lyu, K. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+novel+experience+of+deferential+vessel-sparing+microsurgical+vasoepididymostomy%5BTitle%5D)+AND+%22Asian+Journal+of+Andrology%22%5BJournal%5D)">A novel experience of deferential vessel-sparing microsurgical vasoepididymostomy.</a> Asian Journal of Andrology. 20 (6), 576-580 (2018).</li>
<li>Li, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Vasal+vessel-sparing+microsurgical+single-armed+vasoepididymostomy+to+epididymal+obstructive+azoospermia%3A+A+retrospective+control+study%5BTitle%5D)+AND+%22Andrologia%22%5BJournal%5D)">Vasal vessel-sparing microsurgical single-armed vasoepididymostomy to epididymal obstructive azoospermia: A retrospective control study.</a> Andrologia. 53 (8), 14133(2021).</li>
</ol>

The authors have nothing to disclose.

Vessel-sparing microsurgical vasoepididymostomy might have some clinical significance. Although it is not confirmed that this approach can improve the patency rate, it might better simulate the normal physiological structure and has certain significance for patients who have undergone varicocelectomy. This can be attributed to the fact that preservation of the deferential artery and vas deferens vein have positive effects on the blood supply to the testis after varicocelectomy and the venous return of the testis, respect...

Epididymal obstruction is the most common cause of obstructive azoospermia. Microsurgical vasoepididymostomy (MVE) is the main surgical treatment for epididymal obstruction. Although various MVE techniques1,2,3,4 have been described previously, a two-needle longitudinal intussusception vasoepididymostomy (LIVE) technique described by Chan et al.5,6 has been recognized as the gold standard for achieving a superior patency rate7. The vasal vessels are typically ligated in LIVE. Ligation of the vasal vessels simplifies the procedure and reduces the tension of anastomosis, and a tension-free anastomosis is critical to achieving a successful outcome.
The vas artery primarily supplies the epididymis and vas deferens8. Whether preservation of the vas artery improves the postoperative patency rate remains unclear. However, preserving the vasal vessels during MVE might better simulate the normal physiological structure. Also, for patients who have undergone varicocelectomy, it may be meaningful to perform the vessel-sparing MVE, because the integrity of vasal vasculature plays a vital role in post-varicocelectomy blood supply and the venous return of the testis. A few studies9,10,11 have attempted to preserve the vasal vessels during MVE and suggested that the procedure may have certain benefits. However, preserving the vasal vessels makes the operation of anastomosis more difficult and increases the tension of anastomosis. In addition, the tension of the vas deferens may be transmitted to the vasal vessels and cause postoperative bleeding of the little branches of the vessels. Besides, the existing vessel-sparing procedure may affect the blood supply of the vas deferens. Therefore, we improved the surgical technique of vessel-sparing MVE to make it safer. We called this new procedure microsurgical vasoepididymostomy by preserving the vasal vessels using a retrograde-anterograde dissociation.

<table><tbody><tr><td>11-0 microsutures</td><td>Ningbo Medical Needle Co.,Ltd</td><td>211115</td><td>Double-armed microsurgical nylon suture length: 5 cm</td></tr><tr><td>15&amp;deg; ophthalmic knife</td><td>pearsalls limited</td><td>72-1501</td><td>&amp;nbsp;open the epididymal tubule</td></tr><tr><td>2-0 silk braided non-absorbable suture</td><td>Coated</td><td>1604-51</td><td>Ligation of the vas deferens</td></tr><tr><td>24-Gangiocatheter sheath</td><td>Melsungen AG</td><td>4253523-03</td><td>injection</td></tr><tr><td>5-0 silk braided non-absorbable suture</td><td>Johnson &amp;amp; Johnson</td><td>SA82G</td><td>Ligation of blood vessels</td></tr><tr><td>8-0 microsutures</td><td>Johnson &amp;amp; Johnson</td><td>W2908</td><td>Single-armed microsurgical nylon suture length: 13 cm</td></tr><tr><td>Deferens separating forceps</td><td>Shanghai Medical Instrument Co., Ltd</td><td>JCZ210</td><td>Separation of vas deferens</td></tr><tr><td>Micro scissors</td><td>Shanghai Medical Instrument Co., Ltd</td><td>WA1040</td><td>Microsurgical operation</td></tr><tr><td>Microforceps</td><td>Shanghai Medical Instrument Co., Ltd</td><td>WA3090</td><td>Microsurgical operation</td></tr><tr><td>Microneedle holder</td><td>Shanghai Medical Instrument Co., Ltd</td><td>WA2040</td><td>Microsurgical operation</td></tr><tr><td>Operating microscope</td><td>Leica Microsystems(Sch weiz) AG</td><td>M525MS3</td><td>Microsurgical operation</td></tr></tbody></table>

a modified vessel-sparing microsurgical vasoepididymostomy

A Modified Vessel-Sparing Microsurgical Vasoepididymostomy

Microsurgical vasoepididymostomy (MVE) is the main surgical treatment for epididymal obstruction. The vasal vessels are ligated during MVE. However, preserving the vasal vessels during MVE might better simulate the normal physiological structure and be meaningful for patients who have undergone varicocelectomy. Nevertheless, preserving the vasal vessels might elevate the risk of increasing the tension of anastomosis, affecting the patency rate and leading to delayed postoperative bleeding. Therefore, we developed a novel vessel-sparing MVE to make it safer. Here is a summary of the improvements to the procedure. 1) The retrograde dissociation of the vasal vessels on the proximal testicular side was adopted as the main method, and the anterograde dissociation of the vasal vessels on the distal testicular side was adopted as a supplement to dissociate the vasal vessels to be preserved. This improvement ensures the blood supply to the vas deferens that will be used for anastomosis and also provides longer vasal vessels, which reduces the tension of anastomosis. 2) By fixing the vas deferens to be anastomosed and the broken end of the vas deferens, the free vasal vessels get fixed, which resolves the problem of transmission of vas tension to the vasal vessels and reduces the risk of vasal vessel hemorrhage. 3) Dissociation of the vas deferens after opening the tunica vaginalis increases the mobilization of the vas deferens, which also makes the new procedure easier to complete. The evaluation of the outcomes of this new procedure showed that no significant postoperative complications occurred in the patients, and the patency rate was no different from that of the conventional procedure. Therefore, this new, improved procedure can be considered safe, with satisfactory postoperative results.

A total of 51 patients who underwent vasoepididymostomy at our center between February 2018 and November 2020 were retrospectively analyzed. Considering the anastomotic tension, the modified procedure was performed only in patients with obstruction at the corpus or caudal epididymal, and these patients were included in the current study. Semen examination was performed 1.5 months after the surgery, and the patients were followed up for more than 1 year. Relevant data of patients with the results of at least one semen tes...

Here, we present a protocol to preserve the vasal vessels in microsurgical vasoepididymostomy. The surgical security is enhanced by preserving the vasal vessels using a retrograde-anterograde dissociation and fixing the vasal vessels.

Microsurgical vasoepididymostomy (MVE) is the main surgical treatment for epididymal obstruction. The vasal vessels are ligated during MVE. However, ...

a-modified-vessel-sparing-microsurgical-vasoepididymostomy

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Learn a modified vessel-sparing microsurgical vasoepididymostomy technique for urology and reproductive surgery. This protocol preserves vasal vessels to enhance safety, maintain physiological structures, and ensure high patency rates during fertility restoration procedures.

A Modified Vessel-Sparing Microsurgical Vasoepididymostomy

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