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마이코박테리움 결핵 크기 배제 크로마토그래피를 통한 세포밖 소포 농축
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography
DOI:

00:00 min

May 19, 2022

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Chapters

  • 00:04Introduction
  • 00:50Preparation of Crude Mtb EV Concentrate
  • 02:36Size Exclusion Chromatography for the Enrichment of Mtb EVs from CFP
  • 05:52Quantification and Qualification of the Mtb EVs
  • 07:25Results: Silver Stain and Western Blots by Fraction, TEM Images, and NTA Distribution
  • 08:42Conclusion

Summary

Automatic Translation

이 프로토콜은 크기 배제 크로마토그래피, 배양 상청액으로부터 Mycobacterium tuberculosis 세포밖 소포를 풍부하게하기 위한 용이하고 재현 가능한 기술을 기술한다.

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