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Characterizing Mechanical Properties of Primary Cell Wall in Living Plant Organs Using Atomic Force Microscopy
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Characterizing Mechanical Properties of Primary Cell Wall in Living Plant Organs Using Atomic Force Microscopy
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Characterizing Mechanical Properties of Primary Cell Wall in Living Plant Organs Using Atomic Force Microscopy

DOI:
10.3791/63904-v

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09:52 min

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May 18, 2022

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,
1Laboratory of Plant Cell Growth Mechanisms,Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS

Chapters

  • 00:04Introduction
  • 00:35Sample Preparation for Atomic Force Microscopy Measurements
  • 03:06Data Acquisition
  • 06:40Data Evaluation and Post-Processing
  • 07:26Results: Investigation of Primary Cell Walls of Plant Organs Using Atomic Force Microscopy
  • 09:04Conclusion

Summary

Automatic Translation

Automatic Translation

Studies of cell wall biomechanics are essential for understanding plant growth and morphogenesis. The following protocol is proposed to investigate thin primary cell walls in the internal tissues of young plant organs using atomic force microscopy.

Tags

Keywords: Plant Cell WallCell GrowthBiomechanical CharacterizationAtomic Force MicroscopyVibratome SectioningAgarosePlant OrganNon-fixedNon-dehydratedCell Wall Properties

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