Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Sample Preparation for Rapid Lipid Analysis in…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Sample Preparation for Rapid Lipid Analysis in Drosophila Brain Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
Journal (Biology)
Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
An Assay for Lateral Line Regeneration in Adult Zebrafish
Journal (Biology)
An Assay for Lateral Line Regeneration in Adult Zebrafish
 

Sample Preparation for Rapid Lipid Analysis in Drosophila Brain Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging

Article DOI: 10.3791/63930-v • 09:00 min • July 14th, 2022
July 14th, 2022 •
*1,5,6, *1,2, *3, 1,4, 7, 1,4,5, 1,4,5, 8, 9,10, 2, 1
1Advanced Science Research Center, Neuroscience Initiative, the City University of New York, Graduate Center New York, 2Advanced Science Research Center, Structural Biology Initiative, the City University of New York, Graduate Center, 3National Institute of Neurological Disorders and Stroke, National Institutes of Health, 4The City College of New York, CUNY, 5Macaulay Honors College, CUNY, 6Graduate School of Public Health and Health Policy, The City University of New York, 7Princeton University, 8Ardrey Kell High School, 9The Borough of Manhattan Community College, CUNY, 10Gallatin School of Individualized Study, New York University
* These authors contributed equally

Chapters

Summary
Automatic Translation

The aim of this protocol is to provide detailed guidance on the proper sample preparation for lipid and metabolite analysis in small tissues, such as the Drosophila brain, using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging.

Tags

Lipid Analysis Drosophila Brain Matrix-assisted Laser Desorption/ionization Mass Spectrometry Imaging MALDI-MS Imaging Sample Preparation Lipid Abundance Lipid Distribution In Situ Detection Large Population Analysis Practice Sample Modification Cutting Temperature Cryomold Bubble Formation Dry Ice OCT Solidification Frozen OCT Stages Anesthetizing Flies Carbon Dioxide Petri Dish Laboratory Wipe Static Electricity
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter