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Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
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Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Article DOI: 10.3791/63934-v • 08:58 min • March 3rd, 2023
March 3rd, 2023 •
1, 1
1Antimicrobial Research (AMR) Lab, Department of Biotechnology, University of Mumbai

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Summary
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Here, we present a detailed protocol for the isolation and identification of antibiotic-resistant bacteria from water and the molecular characterization of their antibiotic resistance genes (ARGs). The use of culture-based and non-culture-based (metagenomic analysis) techniques provides complete information about the total bacterial diversity and the total pool of different ARGs present in freshwaters from Mumbai, India.

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Isolation Identification Waterborne Antibiotic-resistant Bacteria Molecular Characterization Antibiotic Resistance Genes Overall Load Bacteria Identity Antibiotic Resistant Traits Antibiotic Resistant Genes Protocol Lab-grown Bacteria Culture-based Techniques Culture-independent Techniques Sewage Sample Pharmaceutical Effluence Sample Hospital Effluence Sample Clinical Settings Sample Nonclinical Settings Sample Experimental Procedures Water Sample Processing Filtering Particulate Matter Removal Serial Dilution Total Bacterial Load Determination
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