Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture

This method helps understand how secretion rates change with time and how cells respond to time varying solid signals. The main advantages of this technique are that its low cost, requires little training and the profusion system setup can be adapted for novel experiments. Begin by filling one syringe with a background solution and load it into the first syringe pump.

Fill another syringe with tracer solution and load it into the second syringe pump. Connect both syringes to two of the three ports of a four-way stopcock using luer connectors. Close the stopcock to the background solution and pump the tracer solution into the stopcock until it drips out the open port.