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Neuroscience

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A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales
 

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales

Article DOI: 10.3791/63941-v
May 10th, 2022

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Summary May 10th, 2022

The protocol provides a detailed method of neuronal imaging in brain slice using a tissue clearing method, ScaleSF. The protocol includes brain tissue preparation, tissue clarification, handling of cleared slices and confocal laser scanning microscopy imaging of neuronal structures from mesoscopic to microscopic levels.

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