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Immunology and Infection

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RNA Fluorescence in situ Hybridization (FISH) to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines
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RNA Fluorescence in situ Hybridization (FISH) to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines

Article DOI: 10.3791/63980-v • 08:58 min • July 27th, 2022
July 27th, 2022 •
1, 2, 2, 1
1Department of Biology, San Diego State University, 2School of Biological Sciences, University of California, San Diego

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Summary
Automatic Translation

Intestinal microbes, including extracellular bacteria and intracellular pathogens like the Orsay virus and microsporidia (fungi), are often associated with wild Caenorhabditis nematodes. This article presents a protocol for detecting and quantifying microbes that colonize and/or infect C. elegans nematodes, and for measuring pathogen load after controlled infections in the lab.

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RNA Fluorescence In Situ Hybridization FISH Microbial Colonization Infection Caenorhabditis Elegans Intestines Visualization Identification Quantification Microbiome Bacteria Intracellular Pathogens Staining Whole Intact Animal Gut Microbiome Bacteria Interactions In Mammalian Systems NGM Plates Microcentrifuge Tubes Washing PBST Fix Nematodes Paraformaldehyde Solution
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