Whole-Kidney Three-Dimensional Staining with CUBIC

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Medicine

JoVE Journal
Medicine

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JoVE Journal Medicine

Whole-Kidney Three-Dimensional Staining with CUBIC

DOI:

10.3791/63986-v

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04:31 min

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July 18, 2022

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Sho Hasegawa, Masaomi Nangaku

¹Division of Nephrology and Endocrinology,The University of Tokyo

Chapters

00:05Introduction
00:37Kidney Fixation, Decolorization, and De-Lipidation
01:45Whole-Mount Immunofluorescent Staining and Refractive Index (RI) Matching
03:03Results: Whole-Mount Immunofluorescent Staining for Three-Dimensional (3D) Kidney Imaging
04:00Conclusion

Summary

Automatic Translation

The present protocol describes a tissue clearing method and whole-mount immunofluorescent staining for three-dimensional (3D) kidney imaging. This technique can offer macroscopic perspectives in kidney pathology, leading to new biological discoveries.

Whole-Kidney Three-Dimensional Staining with CUBIC

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Chapters

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