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体外 坏死性小肠结肠炎的顶端出肠样模型
 
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体外 坏死性小肠结肠炎的顶端出肠样模型

Article DOI: 10.3791/64003-v 09:11 min June 8th, 2022
June 8th, 2022

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Summary

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该协议描述了一种顶端坏死性小肠结肠炎(NEC)在培养皿中的模型,该模型利用具有反向极性的小肠类化合物,允许进入顶端表面。我们提供免疫荧光染色方案来检测NEC相关的上皮破坏,并提供一种确定受NEC培养皿中方案影响的顶出类肠蛋白的活力的方法。

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医学,第184期,
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