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Bioengineering
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Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells
 

Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells

Article DOI: 10.3791/64036-v
September 9th, 2022

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Summary September 9th, 2022

Herein is a protocol for creating dry macroporous alginate scaffolds that mediate efficient viral gene transfer for use in genetic engineering of T cells, including T cells for CAR-T cell therapy. The scaffolds were shown to transduce activated primary T cells with >85% transduction.

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